Characterization of a new Pm2 allele associated with broad-spectrum powdery mildew resistance in wheat line Subtil.
Characterization of a new Pm2 allele associated with broad-spectrum powdery mildew resistance in wheat line Subtil.
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DOI：
10.1038/s41598-017-18827-4
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发表时间：
2018-01-11
影响因子：
4.6
通讯作者：
An D
中科院分区：
文献类型：
--
作者：
Jin Y;Xu H;Ma P;Fu X;Song L;Xu Y;Zhang X;An D
小麦白粉病是一种严重影响产量和品质的病害。结果表明，抗宿主是有效的、环保的。小麦品系Subtil是对30个Bgt分离株中的28个具有抗性的优良种质资源。遗传分析表明，Subtil的白粉病抗性是由一个显性基因赋予的，暂时命名为PmSub。通过大量分离分析，PmSub被定位到染色体臂5DS上，在5.0 cM和0.9 cM处分别有标记Bwm16和Cfd81/Bwm21。等位基因检测进一步证实PmSub与文献记载的Pm2等位基因具有等位基因。然后，从Subtil和Chinese Spring中克隆了Pm2a相关序列的同源序列。与报道的Pm2a序列完全一致，但与中国春的序列有显著差异。根据苏木和中国春同源序列的差异，开发了SWGI067标记。SWGI067与PmSub紧密相连，表明PmSub基因本身与克隆的Pm2a相关序列不同。同时，与其他基因型相比，Subtil与Pm基因在Pm2位点或附近产生了显著不同的反应模式。因此，PmSub极有可能是Pm2的新等位基因。PmSub为小麦的高效改良提供了标记辅助选择的机会。
Wheat powdery mildew is a severe disease affecting yield and quality. Host resistance was proved to be effective and environment-friendly. Wheat line Subtil is an elite germplasm resource resistant to 28 of 30 tested Bgt isolates. Genetic analysis showed that the powdery mildew resistance in Subtil was conferred by a single dominant gene, temporarily designated PmSub. Using bulked segregant analysis, PmSub was mapped to chromosome arm 5DS, and flanked by the markers Bwm16 and Cfd81/Bwm21 at 5.0 and 0.9 cM, respectively. Allelism tests further confirmed PmSub was allelic with documented Pm2 alleles. Then, homologous sequences of Pm2a related sequence was cloned from Subtil and Chinese Spring. It was completely identical to the reported Pm2a sequence, but significantly different from that of Chinese Spring. A marker SWGI067 was developed based on the sequence divergence of homologous sequence in Subtil and Chinese Spring. SWGI067 was closely linked to PmSub, indicating that the gene PmSub itself was different from the cloned Pm2a related sequence. Meanwhile, Subtil produced significantly different reaction pattern compared with other genotypes with Pm genes at or near Pm2 locus. Therefore, PmSub was most likely a new allele of Pm2. PmSub has opportunities for marker-assisted selecting for high-efficiency wheat improvement.
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