Intracerebroventricular Oxytocin Self-Administration in Female Rats.
Intracerebroventricular Oxytocin Self-Administration in Female Rats.
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DOI：
10.1111/jne.12416
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发表时间：
2016-10
影响因子：
3.2
通讯作者：
Wood RI
中科院分区：
文献类型：
--
作者：
Donhoffner ME;Goings SP;Atabaki K;Wood RI
催产素（OT）是一种神经调节剂，促进伴侣关系、母性关怀和社交方式。OT被认为通过增强相关社会刺激的显著性和强化效应来促进这些社会行为。还有一种额外的可能性是，OT本身可能是有益的。为了验证这一点，我们调查了雌性大鼠是否会自愿自我给予OT。雌性Long-Evans大鼠切除卵巢，接受雌激素植入和脑室插管。大鼠在一个有活动和不活动杠杆的操作室中进行测试。他们最初按照固定比例5的计划每天进行4小时的测试，连续5天自行给药人工脑脊液（aCSF），然后再给1或10 ng/ul的aCSF或OT 5天。自用aCSF大鼠产生36.2±6.2个活跃杠杆反应/4h，非活跃杠杆反应14.9±3.4个。对1 ng/ul OT的反应相似。然而，自我给予10 ng/ul OT的大鼠表现出更积极的杠杆反应（67.8±12.0 /4h），并接受121.4±21.0 ng OT/4h。为了确定焦虑的减少是否有助于OT的强化作用，大鼠在进行升高+迷宫测试之前立即接受0.3或3.0 ug的aCSF或OT输注。从张开手臂的时间百分比来看，OT对焦虑没有影响，从闭合手臂进入的次数或总手臂进入的次数来看，OT对运动没有影响。这些结果表明，OT可能是有益的，而这不是由于OT的抗焦虑作用。
Oxytocin (OT) is a neuromodulator that facilitates pair-bonding, maternal care, and social approach. OT is thought to promote these social behaviors by enhancing the salience and reinforcing effects of relevant social stimuli. There is the additional possibility that OT per se may be rewarding. To test this, we investigated whether female rats would voluntarily self-administer OT. Female Long-Evans rats were ovariectomized, and received an estrogen implant and an intracerebroventricular cannula. Rats were tested in an operant chamber with active and inactive levers. They were initially tested 4h/day on a fixed-ratio 5 schedule for self-administration of artificial cerebral spinal fluid (aCSF) for 5 days, followed by aCSF, or OT at 1 or 10 ng/ul for another 5 days. Rats self-administering aCSF made 36.2±6.2 active lever responses/4h vs. 14.9±3.4 inactive responses. Responses for 1 ng/ul OT were similar. However, rats self-administering 10 ng/ul OT made significantly more active lever responses (67.8±12.0 per 4h), and received 121.4±21.0 ng OT/4h. To determine if reduced anxiety contributes to the reinforcing effects of OT, rats received an infusion of aCSF or OT at 0.3 or 3.0 ug immediately before testing on the elevated plus maze. There was no effect of OT on anxiety as reflected by percent time on the open arms, and no effect of OT on locomotion as measured either by the number of closed arm entries or the number of total arm entries. These results suggest that OT may be rewarding, and that this is not due to anxiolytic effects of OT.
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影响因子：
4.8
作者：
BALE, TL;DORSA, DM
通讯作者：
DORSA, DM
影响因子：
2.9
作者：
McCarthy, MM;McDonald, CH;Goldman, D
通讯作者：
Goldman, D
影响因子：
2.9
作者：
ARLETTI, R;BENELLI, A;BERTOLINI, A
通讯作者：
BERTOLINI, A
影响因子：
4.1
作者：
ELLIS, GB;TUREK, FW
通讯作者：
TUREK, FW
影响因子：
2.3
作者：
Gipson, Cassandra D.;Yates, Justin R.;Bardo, Michael T.
通讯作者：
Bardo, Michael T.