Biosynthesis and thermal properties of PHBV produced from levulinic acid by Ralstonia eutropha.
Biosynthesis and thermal properties of PHBV produced from levulinic acid by Ralstonia eutropha.
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富养罗尔斯顿菌乙酰丙酸生产 PHBV 的生物合成及热性质
DOI：
10.1371/journal.pone.0060318
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
He N
中科院分区：
文献类型：
--
作者：
Wang Y;Chen R;Cai J;Liu Z;Zheng Y;Wang H;Li Q;He N
乙酰丙酸（LA）可以从大量可再生的含碳水化合物的生物材料中经济有效地生产。LA可以促进聚合物聚（羟基丁酸酯-co-羟基戊酸酯）（PHBV）和基于PHBV的产品作为碳基质的商业化。因此，本文重点研究了真养罗尔斯通氏菌与LA一起生产PHBV，以产生羟基戊酸（HV），这对增强PHBV的热性能具有重要作用。相应地，在不同浓度的LA下，PHBV的HV含量在0-40.9%之间变化。当向培养物中加入2-6 g L-1 LA时，观察到细胞生长和PHBV积累的刺激。最佳氮源确定为0.5 g L−1氯化铵和2 g L−1酪蛋白胨。确定细胞生长和PHBV积累的最佳pH为7.0。当在30%的低DO浓度和7.0的pH下以大规模（2L发酵罐）进行培养时，获得15.53 g L-1的高最大干细胞重量，其中PHBV浓度为12.61 g L-1（53.9%HV），高达干细胞重量的81.2%。发现PHBV的熔点随着HV在聚合物中存在的分数增加而降低，这导致聚合物的延展性和柔性的改善。本研究的结果将有助于加深对R.真养生物，并且对于生物合成聚合物的工业生产将是有价值的。
Levulinic acid (LA) can be cost-effectively produced from a vast array of renewable carbohydrate-containing biomaterials. LA could facilitate the commercialization of the polymer poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) and PHBV-based products as carbon substrates. Therefore, this paper focused on the production of PHBV by Ralstonia eutropha with LA for hydroxyvalerate (HV) production, which plays an important role in enhancing the thermal properties of PHBV. Accordingly, the HV content of PHBV varied from 0–40.9% at different concentrations of LA. Stimulation of cell growth and PHBV accumulation were observed when 2–6 g L−1 LA was supplied to the culture. The optimal nitrogen sources were determined to be 0.5 g L−1 ammonium chloride and 2 g L−1 casein peptone. It was determined that the optimal pH for cell growth and PHBV accumulation was 7.0. When the cultivation was performed in large scale (2 L fermenter) with a low DO concentration of 30% and a pH of 7.0, a high maximum dry cell weight of 15.53 g L−1 with a PHBV concentration of 12.61 g L−1 (53.9% HV), up to 81.2% of the dry cell weight, was obtained. The melting point of PHBV found to be decreased as the fraction of HV present in the polymer increased, which resulted in an improvement in the ductility and flexibility of the polymer. The results of this study will improve the understanding of the PHBV accumulation and production by R. eutropha and will be valuable for the industrial production of biosynthesized polymers.
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