Characterization of tick organic anion transporting polypeptides (OATPs) upon bacterial and viral infections.
Characterization of tick organic anion transporting polypeptides (OATPs) upon bacterial and viral infections.
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DOI：
10.1186/s13071-018-3160-6
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发表时间：
2018-11-14
影响因子：
3.2
通讯作者：
Neelakanta G
中科院分区：
文献类型：
--
作者：
Taank V;Zhou W;Zhuang X;Anderson JF;Pal U;Sultana H;Neelakanta G
肩突硬蜱有机阴离子转运多肽（OATPs）在蜱-立克次体相互作用中起重要作用。在这份报告中，我们的特点是这些保守分子在感染莱姆病病原体伯氏疏螺旋体或蜱传Langat病毒（LGTV），一种病原体密切相关的蜱传脑炎病毒（TBEV）蜱的作用。实时定量聚合酶链反应分析显示，感染B后，oatps基因表达没有显著变化。未进食的蜱虫体内的伯氏螺旋体在体外用LGTV同步感染未进食的若虫蜱，未发现oatps基因表达的显着变化。然而，特定oatps的表达显着下调LGTV感染蜱细胞在体外。用OATP抑制剂处理蜱细胞显著降低了LGTV负荷、犬尿氨酸氨基转移酶（kat）（一种参与色氨酸代谢物黄尿酸（XA）产生的基因）、蜱细胞中几种oatps的水平和表达。此外，来自一些医学上重要的载体（包括蜱、蚊子和虱）的OATP的生物信息学表征揭示了几个糖基化、磷酸化和肉豆蔻酰化位点的存在。这项研究提供了额外的证据节肢动物OATPs的作用，在载体细胞内病原体相互作用。本文的在线版本（10.1186/s13071-018-3160-6）包含补充材料，可供授权用户使用。
Ixodes scapularis organic anion transporting polypeptides (OATPs) play important roles in tick-rickettsial pathogen interactions. In this report, we characterized the role of these conserved molecules in ticks infected with either Lyme disease agent Borrelia burgdorferi or tick-borne Langat virus (LGTV), a pathogen closely related to tick-borne encephalitis virus (TBEV). Quantitative real-time polymerase chain reaction analysis revealed no significant changes in oatps gene expression upon infection with B. burgdorferi in unfed ticks. Synchronous infection of unfed nymphal ticks with LGTV in vitro revealed no significant changes in oatps gene expression. However, expression of specific oatps was significantly downregulated upon LGTV infection of tick cells in vitro. Treatment of tick cells with OATP inhibitor significantly reduced LGTV loads, kynurenine amino transferase (kat), a gene involved in the production of tryptophan metabolite xanthurenic acid (XA), levels and expression of several oatps in tick cells. Furthermore, bioinformatics characterization of OATPs from some of the medically important vectors including ticks, mosquitoes and lice revealed the presence of several glycosylation, phosphorylation and myristoylation sites. This study provides additional evidence on the role of arthropod OATPs in vector-intracellular pathogen interactions. The online version of this article (10.1186/s13071-018-3160-6) contains supplementary material, which is available to authorized users.
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影响因子：
3.7
作者：
Mitzel, Dana N.;Wolfinbarger, James B.;Bloom, Marshall E.
通讯作者：
Bloom, Marshall E.
影响因子：
9.6
作者：
Mongin, E;Louis, C;Collins, FH
通讯作者：
Collins, FH
影响因子：
6.7
作者：
Neelakanta, Girish;Li, Xin;Fikrig, Erol
通讯作者：
Fikrig, Erol
影响因子：
5.5
作者：
de la Fuente, Jose;Merino, Octavio
通讯作者：
Merino, Octavio
DOI：
10.1056/nejmoa1010493
发表时间：
2011-08-04
期刊：
The New England journal of medicine
影响因子：
--
作者：
Pritt BS;Sloan LM;Johnson DK;Munderloh UG;Paskewitz SM;McElroy KM;McFadden JD;Binnicker MJ;Neitzel DF;Liu G;Nicholson WL;Nelson CM;Franson JJ;Martin SA;Cunningham SA;Steward CR;Bogumill K;Bjorgaard ME;Davis JP;McQuiston JH;Warshauer DM;Wilhelm MP;Patel R;Trivedi VA;Eremeeva ME
通讯作者：
Eremeeva ME