Tension sensing nanoparticles for mechano-imaging at the living/nonliving interface.
Tension sensing nanoparticles for mechano-imaging at the living/nonliving interface.
复制标题
在生活/非生存界面处的张力传感纳米颗粒用于机械成像。
DOI：
10.1021/ja401494e
复制
发表时间：
2013-04-10
影响因子：
15
通讯作者：
Salaita, Khalid
中科院分区：
文献类型：
--
作者：
Liu, Yang;Yehl, Kevin;Narui, Yoshie;Salaita, Khalid
研究界面上的化学-机械耦合对润滑、摩擦学、微流体学和细胞生物学等领域都具有重要意义。一些聚合物宏观和微观系统和悬臂梁已经被开发用于在界面上成像力，但很少有材料适合分子张力传感。为了解决这个问题，我们开发了一种用于分子张力荧光显微镜（MTFM）的金纳米颗粒传感器。作为概念验证，我们以高空间分辨率（<1 μm）成像整合素受体在活细胞和底物之间的界面施加的张力，采集时间为100 ms，具有分子特异性。我们报告的积分素张力值范围为1至15 pN，在局灶粘连中平均为~1 pN。通过使用传统的荧光显微镜，该方法证明了力灵敏度比引力显微镜或PDMS微柱阵列所能达到的力灵敏度高三个数量级，这是细胞生物力学的标准。
Studying chemo-mechanical coupling at interfaces is important for fields ranging from lubrication and tribology to microfluidics and cell biology. Several polymeric macro- and microscopic systems and cantilevers have been developed to image forces at interfaces, but few materials are amenable for molecular tension sensing. To address this issue, we have developed a gold nanoparticle sensor for molecular tension-based fluorescence microscopy (MTFM). As a proof of concept, we imaged the tension exerted by integrin receptors at the interface between living cells and a substrate with high spatial (<1 μm) resolution, at 100 ms acquisition times, and with molecular specificity. We report integrin tension values ranging from 1 to 15 pN and a mean of ~1 pN within focal adhesions. Through the use of a conventional fluorescence microscope, this method demonstrates a force sensitivity that is three orders of magnitude greater than is achievable by traction force microscopy or PDMS micro-post arrays, which are the standard in cellular biomechanics.
登录
查看更多内容
影响因子：
17.1
作者：
Acuna, Guillermo P.;Bucher, Martina;Tinnefeld, Philip
通讯作者：
Tinnefeld, Philip
影响因子：
48
作者：
Stabley, Daniel R.;Jurchenko, Carol;Salaita, Khalid S.
通讯作者：
Salaita, Khalid S.
影响因子：
46.9
作者：
Dubertret, B;Calame, M;Libchaber, AJ
通讯作者：
Libchaber, AJ
影响因子：
64.8
作者：
Chicurel, ME;Singer, RH;Ingber, DE
通讯作者：
Ingber, DE
影响因子：
14.8
作者：
Mei, Bing C.;Susumu, Kimihiro;Mattoussi, Hedi
通讯作者：
Mattoussi, Hedi