Top sheath flow-assisted secondary flow particle manipulation in microchannels with the slanted groove structure
Top sheath flow-assisted secondary flow particle manipulation in microchannels with the slanted groove structure
复制标题
斜槽结构微通道中顶鞘流辅助二次流颗粒操控
DOI：
10.1007/s10404-018-2174-x
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发表时间：
2018
影响因子：
2.8
通讯作者：
Li Weihua
中科院分区：
文献类型：
--
作者：
Zhao Qianbin;Yuan Dan;Tang Shi-Yang;Yun Guolin;Yan Sheng;Zhang Jun;Li Weihua
在惯性微流控领域，二次流被广泛采用来减少颗粒的平衡位数，提高颗粒的聚焦性能。同时，二次流也会增强混合效果，并可能由于诱导旋流而使颗粒聚焦变差，特别是对于较小尺寸的颗粒。在具有斜槽结构的双层微通道中，已经证明了在高流速下产生的二次流可以聚集大尺寸的颗粒(>； 8微米)。然而，由于二次流对小尺寸颗粒的影响不够强，对小颗粒(<； 8微米)的操纵不成功。在这项工作中，为了操纵小尺寸颗粒，我们提出了一种利用顶部鞘流来提高结构诱导二次流的聚焦效率的方案。在较大范围内全面考察了总流量和鞘与样流之间的流量比对实验结果的影响。在适当的鞘流的帮助下，可以在不同的流速下有效地操纵4.8微米的颗粒。此外，还考察了膨胀槽结构的数量、颗粒的浓度和尺寸等因素对颗粒聚焦性能的影响。我们发现，直径为2.9微米的颗粒也可以有效地聚焦在双层微通道中。此外，我们还演示了使用该技术从未稀释的全血中连续提取血浆，以验证其潜在的生物学应用。结果表明，一次提取的血浆纯度可达~ 99%。因此，这表明鞘流助颗粒操纵方法可以克服常规设计的局限性，并为控制更小的颗粒提供更好的性能。这样的平台可以在生物和诊断分析的应用中实现巨大的潜力，用于比正常细胞尺寸小的生物颗粒。
In the regime of inertial microfluidics, the secondary flow is widely adopted to reduce the number of the equilibrium position and improve the focusing performance of particles. At the same time, secondary flow can also enhance the mixing effect and may deteriorate particle focusing due to the induced rotating streams, especially for the particle with a small size. In a double-layered microchannel with slanted groove structures, it has been demonstrated that the generated secondary flow at a high flow rate could focus the particle with a large size (> 8 µm). However, the manipulation of small-size particles (< 8 µm) was unsuccessful as the effects of secondary flow on the small-size particles were not strong enough. In this work, to manipulate the small-size particle, we proposed a scheme to utilize a top sheath flow to enhance the focusing efficiency of the structure-induced secondary flow. The effects of the total flow rate and the flow rate ratio between the sheath and sample flow were investigated comprehensively in a large range. The 4.8 µm particle could be manipulated effectively at different flow rates with the assistance of appropriate sheath flows. Besides, the effects of other factors, such as the quantity of the expansion groove structure, and particle concentration and size, on particle focusing performance were also investigated. We found that the particles with the diameter of 2.9 µm can also be effectively focused within the double-layered microchannel. Furthermore, we demonstrated the continuous plasma extraction from the undiluted whole blood using this proposed technique to validate its potential biological applications. The results show that the purity of plasma extracted could reach up to ~ 99% after a single process. As such, this demonstrated that sheath flow-assisted particle manipulating method can overcome the limitations of conventional design and offer much better performance for controlling smaller particles. Such a platform may enable great potential in the applications of biological and diagnostic assays, for bioparticles smaller than the normal cell size.
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影响因子：
4.1
作者：
Lee, Myung Gwon;Choi, Sungyoung;Park, Je-Kyun
通讯作者：
Park, Je-Kyun
使用带有弧形凹槽阵列的微通道聚焦的高通量鞘和三维微粒。
影响因子：
4.6
作者：
Zhao Q;Zhang J;Yan S;Yuan D;Du H;Alici G;Li W
通讯作者：
Li W
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
Yong‐Seok Choi;K. Seo;Sang-joon Lee
通讯作者：
Yong‐Seok Choi;K. Seo;Sang-joon Lee
影响因子：
6.1
作者：
Haeberle, Stefan;Brenner, Thilo;Ducree, Jens
通讯作者：
Ducree, Jens
影响因子：
3.4
作者：
Hou, Han Wei;Bhagat, Ali Asgar S.;Lim, Chwee Teck
通讯作者：
Lim, Chwee Teck