Evaluation of sustainable susceptibility to Plasmodium vivax infection among colonized Anopheles darlingi and Anopheles deaneorum.
Evaluation of sustainable susceptibility to Plasmodium vivax infection among colonized Anopheles darlingi and Anopheles deaneorum.
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DOI：
10.1186/s12936-022-04204-8
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发表时间：
2022-06-03
期刊：
影响因子：
3
通讯作者：
Araujo, Maisa S.
中科院分区：
文献类型：
--
作者：
Santos, Najara A. C.;Andrade, Alice O.;Santos, Thais C.;Martinez, Leandro N.;Ferreira, Amalia S.;Bastos, Alessandra S.;Martins, Mirilene M.;Pontual, Jose D. C.;Teles, Carolina B. G.;Medeiros, Jansen F.;Araujo, Maisa S.
关键词：
通过直接的膜进食测定（DMFA）易于疟原虫的蚊子的定殖有可能显着提高我们对Vivax生物学，载体 - 寄生虫相互作用和透射阻滞疫苗的了解。自2018年以来，西部巴西亚马逊的疟疾一直保持着这些物种的殖民地当建立菌落时，确认了疟原虫的易感性，但需要维持这些菌落的敏感性，以使其对病原体传播保持良好的模型。西部亚马逊。
F10-F25世代的实验室蚊子通过DMFA喂食。通过DMFA，通过血液喂养后7天用卵囊负荷表示的感染率和强度来确定易感性从初始血液喂养中评估了感染对蚊子生存的影响，直到比较蚊子之间的孢子形成率和生存率在感染和未感染的血液中喂养。
在物种的患病率和感染的强度中，伴随着感染的蚊子的差异很大。 ）观察到配子细胞血症和感染的患病率/强度之间，但是卵囊和孢子岩负荷具有现代与强相关。
Darlingi殖民地对病原体的传播产生了出色的主题。预测Vivax的蚊子感染，但应使用卵囊强度来安排Sporozoite实验。
在线版本中包含10.1186/s12936-022-04204-8的补充材料。
The colonization of mosquitoes susceptible to Plasmodium vivax via direct membrane feeding assay (DMFA) has the potential to significantly advance our knowledge of P. vivax biology, vector-parasite interaction and transmission-blocking vaccine research. Anopheles darlingi and Anopheles deaneorum are important vectors of malaria in the Western Brazilian Amazon. Since 2018, well-established colonies of these species have been maintained in order to mass produce mosquitoes destined for P. vivax infection. Plasmodium susceptibility was confirmed when the colonies were established, but susceptibility needs to be maintained for these colonies to remain good models for pathogen transmission. Thus, the susceptibility was assessed of colonized mosquitoes to P. vivax isolates circulating in the Western Amazon. Laboratory-reared mosquitoes from F10-F25 generations were fed on P. vivax blood isolates via DMFA. Susceptibility was determined by prevalence and intensity of infection as represented by oocyst load seven days after blood feeding, and sporozoite load 14 days after blood feeding. The effect of infection on mosquito survival was evaluated from initial blood feeding until sporogonic development and survival rates were compared between mosquitoes fed on infected and uninfected blood. Correlation was calculated between gametocytaemia and prevalence/intensity of infection, and between oocyst and sporozoite load. Significant differences were found in prevalence and intensity of infection between species. Anopheles darlingi showed a higher proportion of infected mosquitoes and higher oocyst and sporozoite intensity than An. deaneorum. Survival analysis showed that An. deaneorum survival decreased drastically until 14 days post infection (dpi). Plasmodium vivax infection decreased survival in both species relative to uninfected mosquitoes. No correlation was observed between gametocytaemia and prevalence/intensity of infection, but oocyst and sporozoite load had a moderate to strong correlation. Colonized An. darlingi make excellent subjects for modelling pathogen transmission. On the other hand, An. deaneorum could serve as a model for immunity studies due the low susceptibility under current colonized conditions. In the application of DMFA, gametocyte density is not a reliable parameter for predicting mosquito infection by P. vivax, but oocyst intensity should be used to schedule sporozoite experiments. The online version contains supplementary material available at 10.1186/s12936-022-04204-8.
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影响因子：
3
作者：
Andolina C;Landier J;Carrara V;Chu CS;Franetich JF;Roth A;Rénia L;Roucher C;White NJ;Snounou G;Nosten F
通讯作者：
Nosten F
DOI：
10.1016/j.ijpddr.2021.09.003
发表时间：
2021-12
期刊：
International journal for parasitology. Drugs and drug resistance
影响因子：
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作者：
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通讯作者：
Krettli AU
影响因子：
9.6
作者：
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通讯作者：
Read, AF
影响因子：
2.1
作者：
Araujo, Maisa da Silva;Andrade, Alice Oliveira;Pereira-da-Silva, Luiz Hidelbrando
通讯作者：
Pereira-da-Silva, Luiz Hidelbrando
影响因子：
34.3
作者：
Brasil, Patricia;Zalis, Mariano Gustavo;Daniel-Ribeiro, Claudio Tadeu
通讯作者：
Daniel-Ribeiro, Claudio Tadeu