Common Lung Microbiome Identified among Mechanically Ventilated Surgical Patients.
Common Lung Microbiome Identified among Mechanically Ventilated Surgical Patients.
复制标题
DOI：
10.1371/journal.pone.0166313
复制
发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Allen MS
中科院分区：
文献类型：
--
作者：
Smith AD;Zhang Y;Barber RC;Minshall CT;Huebinger RM;Allen MS
非慢性疾病患者肺微生物组的检查尚未得到广泛的研究。传统的基于培养的筛选方法往往无法从支气管肺泡灌洗液样本中识别细菌。新一代测序技术的进步允许一个培养独立的分子为基础的分析，以确定该患者群体的肺部微生物组成。在本研究中，使用Ion Torrent PGM系统评估培养阴性支气管肺泡灌洗液样品的微生物复杂性。鉴定出一组样品，所有样品都显示出高度多样性和相似的细菌相对丰度。该组包括食氢菌、未分类拟杆菌门、Pedobacter、Thauera和不动杆菌门。这些细菌可能是该患者群体中常见的非致病性肺微生物群的代表。
The examination of the pulmonary microbiome in patients with non-chronic disease states has not been extensively examined. Traditional culture based screening methods are often unable to identify bacteria from bronchoalveolar lavage samples. The advancement of next-generation sequencing technologies allows for a culture-independent molecular based analysis to determine the microbial composition in the lung of this patient population. For this study, the Ion Torrent PGM system was used to assess the microbial complexity of culture negative bronchoalveolar lavage samples. A group of samples were identified that all displayed high diversity and similar relative abundance of bacteria. This group consisted of Hydrogenophaga, unclassified Bacteroidetes, Pedobacter, Thauera, and Acinetobacter. These bacteria may be representative of a common non-pathogenic pulmonary microbiome associated within this population of patients.
登录
查看更多内容
影响因子：
14.2
作者：
Marri, Pradeep Reddy;Stern, Debra A.;Wright, Anne L.;Billheimer, Dean;Martinez, Fernando D.
通讯作者：
Martinez, Fernando D.
影响因子：
3.7
作者：
Erb-Downward JR;Thompson DL;Han MK;Freeman CM;McCloskey L;Schmidt LA;Young VB;Toews GB;Curtis JL;Sundaram B;Martinez FJ;Huffnagle GB
通讯作者：
Huffnagle GB
DOI：
10.1164/rccm.201210-1913oc
发表时间：
2013-05-15
影响因子：
24.7
作者：
Morris, Alison;Beck, James M.;Weinstock, George M.
通讯作者：
Weinstock, George M.
DOI：
10.1097/01.ta.0000220424.34835.f1
发表时间：
2006-05-01
影响因子：
--
作者：
Minei, Joseph P.;Nathens, Avery B.;Maier, Ronald V.
通讯作者：
Maier, Ronald V.
影响因子：
4.4
作者：
Schloss, Patrick D.;Westcott, Sarah L.;Weber, Carolyn F.
通讯作者：
Weber, Carolyn F.