Genetic tagging of active neurons in auditory cortex reveals maternal plasticity of coding ultrasonic vocalizations.
Genetic tagging of active neurons in auditory cortex reveals maternal plasticity of coding ultrasonic vocalizations.
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DOI：
10.1038/s41467-018-03183-2
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发表时间：
2018-02-28
影响因子：
16.6
通讯作者：
Mizrahi A
中科院分区：
文献类型：
--
作者：
Tasaka GI;Guenthner CJ;Shalev A;Gilday O;Luo L;Mizrahi A
即使是分子定义的亚型，皮质神经元在功能上也常常是异质的。在感觉皮质中，对自然刺激的生理反应可能是稀疏的，甚至相邻的神经元也会有很大的差异。因此，很难解析出为进一步实验编码特定刺激的电路。在这里，我们报道了一种cre报告小鼠的发展，该小鼠允许重组细胞标记和基因操作，并将其与活动依赖的Fos-CreERT2驱动程序一起使用，以识别听觉皮层中的功能活跃回路。体内靶向贴片记录验证了我们的神经元对生理相关的自然声音（如小狗蠕动的叫声和超声波发声（usv））做出反应的方法。使用该系统研究产后母亲的皮质反应，我们发现神经元的短暂募集对usv高度敏感。这个神经元亚群具有独特的生理特性，可以提高幼犬USV呼叫的编码效率，这意味着它是亲代可塑性的独特特征。Fos-CreER敲入小鼠能够标记在特定时间窗口内激活的神经元。在这里，作者开发了一种低基线激活的Cre报告小鼠，并用它来揭示幼年小鼠和母亲的听觉皮层神经元被幼鼠叫声激活的特定编码特性。
Cortical neurons are often functionally heterogeneous even for molecularly defined subtypes. In sensory cortices, physiological responses to natural stimuli can be sparse and vary widely even for neighboring neurons. It is thus difficult to parse out circuits that encode specific stimuli for further experimentation. Here, we report the development of a Cre-reporter mouse that allows recombination for cellular labeling and genetic manipulation, and use it with an activity-dependent Fos-CreERT2 driver to identify functionally active circuits in the auditory cortex. In vivo targeted patch recordings validate our method for neurons responding to physiologically relevant natural sounds such as pup wriggling calls and ultrasonic vocalizations (USVs). Using this system to investigate cortical responses in postpartum mothers, we find a transient recruitment of neurons highly responsive to USVs. This subpopulation of neurons has distinct physiological properties that improve the coding efficiency for pup USV calls, implicating it as a unique signature in parental plasticity. Fos-CreER knock-in mice enable tagging of neurons that are activated within a distinct time window. Here, the authors develop a Cre reporter mouse with low baseline activation and use it to reveal the specific coding properties of auditory cortex neurons that are activated by pup calls in both naive mice and mothers.
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影响因子：
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作者：
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通讯作者：
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