Seasonal variations in bacterial communities and antibiotic-resistant strains associated with green bottle flies (Diptera: Calliphoridae)
Seasonal variations in bacterial communities and antibiotic-resistant strains associated with green bottle flies (Diptera: Calliphoridae)
复制标题
与绿瓶蝇（双翅目：丽蝇科）相关的细菌群落和抗生素抗性菌株的季节性变化
DOI：
10.1007/s00253-013-5498-1
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Y. Tanji
中科院分区：
文献类型：
--
作者：
Ting Wei;R. Ishida;K. Miyanaga;Y. Tanji
绿色瓶蝇在日本的人类环境中频繁发生。许多种绿色瓶蝇在法医检验或临床治疗中的重要性已被研究，但与这组苍蝇相关的细菌群落尚未得到全面调查。本研究利用454焦磷酸测序技术对不同季节采集的绿色瓶蝇体内的细菌群落进行了研究。同时，用选择性培养基筛选细菌并进行药物敏感性试验。在三个不同的季节收集的样品窝藏独特的细菌群落。春季与绿色瓶蝇相关的优势属为葡萄球菌属，夏季为Ignatzschinerians属，秋季为迷走球菌属、Dysgonomonas属和一种未分类的醋球菌属。从春季到秋季，细菌群落多样性呈上升趋势。气候条件的变化可能是这些季节性变化的原因在苍蝇相关的细菌群落。不同季节分离出的耐药细菌种类也不同，但很难将耐药细菌的季节性变化与整个群落的变化联系起来。分离出一批多重耐药菌，其中部分菌株与粪肠球菌、屎肠球菌等病原菌密切相关，对公共卫生构成严重威胁。总体而言，这项研究为我们提供了有关绿色瓶蝇中细菌群落的组成和季节性的信息，并强调了苍蝇介导的耐药病原体传播的风险。
Green bottle flies occur frequently around human environments in Japan. Many species of green bottle flies have been studied with regard to their importance in forensic examinations or clinical therapies, but the bacterial communities associated with this group of flies have not been comprehensively investigated. In this research, 454 pyrosequencing was used to reveal the bacterial communities in green bottle flies collected in different seasons. Meanwhile, the bacteria were screened with selective media and tested for antibiotic susceptibility. Samples collected in three different seasons harbored distinctive bacterial communities. The predominant genera associated with green bottles flies wereStaphylococcusin spring,Ignatzschineriain summer, andVagococcus,Dysgonomonas, and an unclassifiedAcetobacteraceaein autumn. An upward trend in bacterial community diversity was observed from spring to autumn. Changes in climatic conditions could be the cause of these seasonal variations in fly-associated bacterial communities. The species of isolated antibiotic-resistant bacteria also differed across seasons, but it was difficult to correlate seasonal changes in antibiotic-resistant bacteria with changes in whole communities. A number of multiple-antibiotic-resistant bacteria were isolated, and some of these strains were closely affiliated with pathogens such asEnterococcus faecalisandEnterococcus faecium, which could cause serious threats to public health. Overall, this research provided us with information about the composition and seasonality of bacterial communities in green bottle flies, and highlighted the risks of fly-mediated dissemination of antibiotic-resistant pathogens.
登录
查看更多内容
DOI：
10.1099/00207713-51-2-401
发表时间：
2001-03-01
影响因子：
2.8
作者：
Tóth, E;Kovács, G;Márialigeti, K
通讯作者：
Márialigeti, K
影响因子：
5
作者：
Tingting Wei;Jun Hu;K. Miyanaga;Y. Tanji
通讯作者：
Tingting Wei;Jun Hu;K. Miyanaga;Y. Tanji
人工树林中绿蝇和其他昆虫的季节性丰度和垂直迁徙。
影响因子：
2.3
作者：
M. Townsend
通讯作者：
M. Townsend
影响因子：
5
作者：
Kaoruko Seino;T. Takano;Keiko Nakamura;Masafumi Watanabe
通讯作者：
Kaoruko Seino;T. Takano;Keiko Nakamura;Masafumi Watanabe
影响因子：
30.3
作者：
Cirimotich CM;Ramirez JL;Dimopoulos G
通讯作者：
Dimopoulos G