Improving the population genetics toolbox for the study of the African malaria vector Anopheles nili: microsatellite mapping to chromosomes.
Improving the population genetics toolbox for the study of the African malaria vector Anopheles nili: microsatellite mapping to chromosomes.
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DOI：
10.1186/1756-3305-4-202
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发表时间：
2011-10-19
影响因子：
3.2
通讯作者：
Sharakhov IV
中科院分区：
文献类型：
--
作者：
Peery A;Sharakhova MV;Antonio-Nkondjio C;Ndo C;Weill M;Simard F;Sharakhov IV
尼利按蚊是撒哈拉以南非洲潮湿的稀树草原和森林地区疟疾的主要传播媒介。了解该物种的种群遗传结构和进化动态对于非洲制定适当且有针对性的疟疾控制战略非常重要。染色体倒位和微卫星标记通常用于研究疟疾蚊子的种群结构。将这些标记物理映射到染色体上进一步改进了工具箱，并可以推断目标物种的人口统计和进化历史。多线染色体的可用性使我们能够开发微卫星标记图谱并研究染色体倒位的多态性。九个微卫星标记被映射到An的所有五个染色体臂上的独特位置。 nili 使用荧光原位杂交 (FISH)。探针从质粒克隆的 300-483 bp 长插入片段和使用 An 设计的引物扩增的 506-559 bp 长片段中获得。在 Illumina 平台上生成的 nili 基因组组装。另外两个基因座被分配给 An 的特定染色体臂。 nili 基于与冈比亚按蚊的计算机序列相似性和染色体同线性。三个微卫星被定位在多态性染色体倒位 2Rb 和 2Rc 的内部或附近。在天然 An 中检测到，由于 2Rb 倒位杂合子的缺陷，以及两个倒位之间高度显着的连锁不平衡，在统计学上显着偏离了 Hardy-Weinberg 平衡。从布基纳法索收集的尼利种群。我们的研究表明，下一代测序可用于改进 FISH，在没有参考基因组序列的物种中进行微卫星定位。 An 中微卫星标记的物理图谱。 nili 表明它们的细胞学位置跨越整个五臂互补体，从而允许进行全基因组推断。关于微卫星标记的多态性倒位和染色体位置的知识有助于解释基因座间遗传变异的差异以及按蚊自然群体中观察到的显着差异。尼利。
Anopheles nili is a major vector of malaria in the humid savannas and forested areas of sub-Saharan Africa. Understanding the population genetic structure and evolutionary dynamics of this species is important for the development of an adequate and targeted malaria control strategy in Africa. Chromosomal inversions and microsatellite markers are commonly used for studying the population structure of malaria mosquitoes. Physical mapping of these markers onto the chromosomes further improves the toolbox, and allows inference on the demographic and evolutionary history of the target species. Availability of polytene chromosomes allowed us to develop a map of microsatellite markers and to study polymorphism of chromosomal inversions. Nine microsatellite markers were mapped to unique locations on all five chromosomal arms of An. nili using fluorescent in situ hybridization (FISH). Probes were obtained from 300-483 bp-long inserts of plasmid clones and from 506-559 bp-long fragments amplified with primers designed using the An. nili genome assembly generated on an Illumina platform. Two additional loci were assigned to specific chromosome arms of An. nili based on in silico sequence similarity and chromosome synteny with Anopheles gambiae. Three microsatellites were mapped inside or in the vicinity of the polymorphic chromosomal inversions 2Rb and 2Rc. A statistically significant departure from Hardy-Weinberg equilibrium, due to a deficit in heterozygotes at the 2Rb inversion, and highly significant linkage disequilibrium between the two inversions, were detected in natural An. nili populations collected from Burkina Faso. Our study demonstrated that next-generation sequencing can be used to improve FISH for microsatellite mapping in species with no reference genome sequence. Physical mapping of microsatellite markers in An. nili showed that their cytological locations spanned the entire five-arm complement, allowing genome-wide inferences. The knowledge about polymorphic inversions and chromosomal locations of microsatellite markers has been useful for explaining differences in genetic variability across loci and significant differentiation observed among natural populations of An. nili.
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影响因子：
2.7
作者：
Goltsev, Yury;Rezende, Gustavo L.;Vranizan, Karen;Lanzaro, Greg;Valle, Denise;Levine, Michael
通讯作者：
Levine, Michael
影响因子：
2.1
作者：
Antonio-Nkondjio, C;Awono-Ambene, P;Fontenille, D
通讯作者：
Fontenille, D
影响因子：
3.3
作者：
Cohuet, A;Dia, I;Fontenille, D
通讯作者：
Fontenille, D
DOI：
10.1046/j.1471-8286.2003.00462.x
发表时间：
2003-09-01
期刊：
MOLECULAR ECOLOGY NOTES
影响因子：
--
作者：
Berthomieu, A;Kengne, P;Weill, M
通讯作者：
Weill, M
影响因子：
1.9
作者：
CARNEVALE, P;LEGOFF, G;ROBERT, V
通讯作者：
ROBERT, V