Expression of vascular endothelial growth factor and E-cadherin in human ovarian cancer: association with ascites fluid accumulation and peritoneal dissemination in mouse ascites model.
Expression of vascular endothelial growth factor and E-cadherin in human ovarian cancer: association with ascites fluid accumulation and peritoneal dissemination in mouse ascites model.
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DOI：
10.1111/j.1349-7006.2002.tb01302.x
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发表时间：
2002-06
期刊：
影响因子：
--
通讯作者：
Kudo R
中科院分区：
文献类型：
--
作者：
Akutagawa N;Nishikawa A;Iwasaki M;Fujimoto T;Teramoto M;Kitajima Y;Endo T;Shibuya M;Kudo R
腹水形成和腹膜转移是晚期卵巢癌患者的关键问题。血管内皮生长因子（VEGF），也称为血管生成生长因子，是腹腔积液和肿瘤血管生成的有效介质。E-钙粘蛋白是一种粘附分子，对细胞间相互作用很重要。为了阐明卵巢癌腹水形成和腹膜播散的分子机制，我们检测了VEGF和E‐cadherin在不同卵巢癌细胞系中的表达，并利用裸鼠比较卵巢癌细胞的生物学特性。本研究中使用了三种人卵巢癌细胞系（AMOC-2、HNOA和HTBOA）。通过北方印迹和RT-PCR方法分析基因表达。AMOC-2表达E-cadherin，但不表达VEGF。HNOA表达VEGF而不表达E-钙粘蛋白。HTBOA同时表达VEGF和E-钙粘蛋白。每个人类卵巢癌模型都揭示了一个特定的特征。AMOC-2小鼠具有单个大的腹膜肿瘤，没有腹水或显著的腹膜扩散。HTBOA和HNOA小鼠有血性腹水和明显的腹膜播散。VEGF反义核酸转染HTBOA细胞后，腹水形成明显减少。提示VEGF通过增加血管通透性作用在腹水形成中起重要作用。E-cadherin表达的失调可能参与了腹膜播散。这些分子对于形成晚期卵巢癌的特定特征是重要的。具有不同基因表达模式的卵巢癌细胞系产生具有不同特征的裸鼠人卵巢癌模型。
Ascites formation and peritoneal dissemination are critical problems in patients with advanced ovarian cancer. Vascular endothelial growth factor (VEGF), also known as angiogenic growth factor, is a potent mediator of peritoneal fluid accumulation and angiogenesis of tumors. E‐Cadherin is an adhesion molecule that is important for cell‐to‐cell interaction. To elucidate the molecular mechanism of ascites formation and peritoneal dissemination of ovarian cancer, we examined the expression of VEGF and E‐cadherin in different ovarian cancer cell lines and utilized nude mice to compare the biological characteristics of ovarian cancer cells. Three human ovarian cancer cell lines (AMOC‐2, HNOA and HTBOA) were used in this study. Expression of genes was analyzed by northern blotting and RT‐PCR methods. AMOC‐2 expressed E‐cadherin, but not VEGF. HNOA expressed VEGF without E‐cadherin expression. HTBOA expressed both VEGF and E‐cadherin. Each human ovarian cancer model revealed a specific feature. The AMOC‐2 mouse had a single large peritoneal tumor without ascites or remarkable peritoneal dissemination. HTBOA and HNOA mice had bloody ascites and marked peritoneal dissemination. Introduction of VEGF antisense into HTBOA cells could inhibit the ascites formation. It is suggested that VEGF is important for the ascites formation via the increased vascular permeability effect. The deregulation of E‐cadherin expression might be involved in the peritoneal dissemination. These molecules are important for the formation of specific features of advanced ovarian cancer. Ovarian cancer cell lines that had different gene expression patterns produced nude mouse human ovarian cancer models with different characteristics.
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影响因子：
8.8
作者：
Matsuura, K;Kawanishi, J;Fujii, S;Imamura, M;Hirano, S;Takeichi, M;Niitsu, Y
通讯作者：
Niitsu, Y
影响因子：
4.7
作者：
Nishikawa, A;Iwasaki, M;Kudo, R
通讯作者：
Kudo, R
影响因子：
8.8
作者：
Sato F;Shimada Y;Watanabe G;Uchida S;Makino T;Imamura M
通讯作者：
Imamura M
DOI：
10.1083/jcb.111.5.2077
发表时间：
1990-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Musil LS;Cunningham BA;Edelman GM;Goodenough DA
通讯作者：
Goodenough DA
影响因子：
3.3
作者：
BERSE, B;BROWN, LF;SENGER, DR
通讯作者：
SENGER, DR