Identification and fine mapping of qPH6, a novel major quantitative trait locus for plant height in rice

Identification and fine mapping of qPH6, a novel major quantitative trait locus for plant height in rice
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水稻株高主要数量性状基因座qPH6的鉴定与精细定位

DOI:
10.1007/s11032-015-0196-6
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发表时间:
2015-01
期刊:
影响因子:
3.1
通讯作者:
Guohua Liang
Guohua Liang
中科院分区:
农林科学2区
文献类型:
--
作者:
Yuan Yuan;Jun Miao;Yajun Tao;Chaoqiu Ji;Peina Du;Jun Wang;Zhongde Wang;Da Chen;Zhiyun Gong;Chu;eng Yi;Jinyan Zhu;Guichun Dong;Minghong Gu;Yong Zhou;Guohua Liang

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株高是影响水稻株型和产量的决定因素之一。发掘与株高相关的新基因是水稻高产育种的必要条件。本研究以粳稻品种(Balilla)为轮回亲本,籼稻品种(Dular)为供体亲本的回交导入系C33084为材料,研究了一个对水稻株高有较大影响的新的主要数量性状基因座。C33084的株高显著高于Balilla。 来自Balilla的隐性等位基因qph 6对株高有负效应。对F2和F4代共1,791个隐性个体进行了精细定位,并根据现有序列注释数据库将该基因定位到包含3个注释基因的~41-kb区域。这些信息可能有助于未来的候选基因的鉴定和分离。对近等基因系NIL(qPH 6)和NIL(qph 6)的表型分析表明,qPH 6对其它性状没有多效效应。qPH 6基因的发现可能有助于高产优质作物的设计和育种。
Plant height is one of the determinants that influence rice plant architecture and grain yield. Identification of useful novel genes related to plant height is necessary for breeding high-yield rice. In this study, C33084, a backcross introgression line derived from a  japonica cultivar (Balilla) as the recurrent parent and an indica cultivar (Dular) as the donor parent, was employed to characterize a novel major quantitative trait locus with a large effect on plant height in rice. C33084 showed a significantly higher plant height than Balilla. The recessive allele, qph6, from Balilla has a negative effect on plant height. Fine mapping was carried out with a total of 1,791 recessive individuals from the F2 and F4 generations, and the gene was delimited to a ~41-kb region containing three annotated genes according to available sequence annotation databases. This information could be helpful for future identification and isolation of the candidate genes. Phenotypic evaluation of the near isogenic lines NIL (qPH6) and NIL (qph6) suggested that qPH6 has no pleiotropic effect on other traits. The discovery of qPH6 might be useful for the design and breeding of crops with high grain yield and quality.
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