Oral Microbial Species and Virulence Factors Associated with Oral Squamous Cell Carcinoma.
Oral Microbial Species and Virulence Factors Associated with Oral Squamous Cell Carcinoma.
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DOI：
10.1007/s00248-020-01596-5
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发表时间：
2021-11
影响因子：
3.6
通讯作者：
Nelson KE
中科院分区：
文献类型：
--
作者：
Torralba MG;Aleti G;Li W;Moncera KJ;Lin YH;Yu Y;Masternak MM;Golusinski W;Golusinski P;Lamperska K;Edlund A;Freire M;Nelson KE
人类微生物组一直是许多研究工作的重点，以阐明包括癌症在内的人类疾病的发病机制。与其他癌症相比，口腔癌的死亡率很高，因为诊断通常是在晚期。在过去的十年中，它在美国的患病率有所上升，每年有超过40,000名新的癌症患者。此外，口腔癌的发病机制尚不完全清楚，可能是多因素的。为了揭示与口腔微生物组及其毒力因子的关系，我们使用16S rDNA和宏基因组测序来表征来自18名口腔鳞状细胞癌（OSCC）患者的肿瘤组织、非肿瘤组织和唾液中的微生物组成和功能含量。结果表明，与所有其他样本类型相比，与肿瘤组织相关的梭杆菌门、拟杆菌门和厚壁菌门的细菌数量更多。此外，唾液宏蛋白质组学显示普雷沃氏菌在5名OSCC受试者中显著增加，而棒状杆菌在10名健康受试者中主要增加。最后，我们确定了与戈多氏链球菌和已知口腔病原体相关的粘附和毒力因子，这些口腔病原体属于梭杆菌属，主要存在于OSCC组织中。根据这些结果，我们提出，本研究中使用的方法不仅可以大大提高OSCC的诊断，而且在肿瘤组织中检测到的来自该门的生物体和特定毒力因子可能是表征疾病进展的优秀生物标志物。本文的在线版本（10.1007/s00248-020-01596-5）包含补充资料，仅供授权用户使用。
The human microbiome has been the focus of numerous research efforts to elucidate the pathogenesis of human diseases including cancer. Oral cancer mortality is high when compared with other cancers, as diagnosis often occurs during late stages. Its prevalence has increased in the USA over the past decade and accounts for over 40,000 new cancer patients each year. Additionally, oral cancer pathogenesis is not fully understood and is likely multifactorial. To unravel the relationships that are associated with the oral microbiome and their virulence factors, we used 16S rDNA and metagenomic sequencing to characterize the microbial composition and functional content in oral squamous cell carcinoma (OSCC) tumor tissue, non-tumor tissue, and saliva from 18 OSCC patients. Results indicate a higher number of bacteria belonging to the Fusobacteria, Bacteroidetes, and Firmicutes phyla associated with tumor tissue when compared with all other sample types. Additionally, saliva metaproteomics revealed a significant increase of Prevotella in five OSCC subjects, while Corynebacterium was mostly associated with ten healthy subjects. Lastly, we determined that there are adhesion and virulence factors associated with Streptococcus gordonii as well as from known oral pathogens belonging to the Fusobacterium genera found mostly in OSCC tissues. From these results, we propose that not only will the methods utilized in this study drastically improve OSCC diagnostics, but the organisms and specific virulence factors from the phyla detected in tumor tissue may be excellent biomarkers for characterizing disease progression. The online version of this article (10.1007/s00248-020-01596-5) contains supplementary material, which is available to authorized users.
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影响因子：
6.4
作者：
Al-Hebshi NN;Li S;Nasher AT;El-Setouhy M;Alsanosi R;Blancato J;Loffredo C
通讯作者：
Loffredo C
影响因子：
3.1
作者：
Dorn, BR;Leung, KP;Progulske-Fox, A
通讯作者：
Progulske-Fox, A
影响因子：
7.6
作者：
Furquim, C. P.;Soares, G. M. S.;Teles, F. R. F.
通讯作者：
Teles, F. R. F.
影响因子：
7
作者：
Castellarin, Mauro;Warren, Rene L.;Holt, Robert A.
通讯作者：
Holt, Robert A.
犬口腔微生物组。
影响因子：
3.7
作者：
Dewhirst FE;Klein EA;Thompson EC;Blanton JM;Chen T;Milella L;Buckley CM;Davis IJ;Bennett ML;Marshall-Jones ZV
通讯作者：
Marshall-Jones ZV