Catecholamines promote Actinobacillus pleuropneumoniae growth by regulating iron metabolism.
Catecholamines promote Actinobacillus pleuropneumoniae growth by regulating iron metabolism.
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DOI：
10.1371/journal.pone.0121887
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Zhou R
中科院分区：
文献类型：
--
作者：
Li L;Chen Z;Bei W;Su Z;Huang Q;Zhang L;Chen H;Zhou R
儿茶酚胺是一种宿主应激激素，可通过促进铁的利用和/或通过特定的激素受体调节毒力基因的表达来诱导许多细菌的生长。这两条反应通路是否相互关联尚不得而知。在我们前期的研究中发现，儿茶酚胺可以调节猪呼吸道重要病原体胸膜肺炎放线杆菌大量基因的表达。儿茶酚胺不影响细菌在富营养液中的生长。在这项研究中，发现儿茶酚胺影响胸膜肺炎沙门氏菌在化学定义培养基(CDM)中的生长。我们发现，在CDM中，血清抑制胸膜肺炎沙门氏菌的生长，而肾上腺素、去甲肾上腺素和多巴胺在含有CDM的血清中促进胸膜肺炎沙门氏菌的生长。已知的细菌激素受体QSec在这一过程中没有发挥作用。离子补充和转录组分析表明，血清添加导致铁限制条件，而儿茶酚胺的添加缓解了这种情况。血清中的转铁蛋白抑制CDM中胸膜肺炎沙门氏菌的生长，这种作用可被TonB2依赖的儿茶酚胺逆转。我们的数据表明，儿茶酚胺通过调节铁的获取和代谢促进胸膜肺炎沙门氏菌的生长，这种作用不依赖于肾上腺素能受体QSec。
Catecholamines are host stress hormones that can induce the growth of many bacteria by facilitating iron utilization and/or regulate the expression of virulence genes through specific hormone receptors. Whether these two responsive pathways are interconnected is unknown. In our previous study, it was found that catecholamines can regulate the expression of a great number of genes of Actinobacillus pleuropneumoniae, an important swine respiratory pathogen. However, bacterial growth was not affected by catecholamines in rich medium. In this study, it was discovered that catecholamines affected A. pleuropneumoniae growth in chemically defined medium (CDM). We found that serum inhibited A. pleuropneumoniae growth in CDM, while epinephrine, norepinephrine and dopamine promoted A. pleuropneumoniae growth in the CDM containing serum. The known bacterial hormone receptor QseC didn’t play roles in this process. Ion-supplementation and transcriptome analysis indicated that serum addition resulted in iron-restricted conditions which were alleviated by the addition of catecholamines. Transferrin, one of the components in serum, inhibited the growth of A. pleuropneumoniae in CDM, an effect reversed by addition of catecholamines in a TonB2-dependent manner. Our data demonstrate that catecholamines promote A. pleuropneumoniae growth by regulating iron-acquisition and metabolism, which is independent of the adrenergic receptor QseC.
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影响因子：
3.2
作者：
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通讯作者：
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影响因子：
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大肠杆菌O157：H7，沙门氏菌和耶尔森氏菌小肠结肠炎的肾上腺素能和多巴胺能受体拮抗剂对儿茶酚胺诱导的肾上腺素能受体拮抗剂的封锁。
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影响因子：
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作者：
Bearson, Bradley L.;Bearson, Shawn M. D.;Lay, Donald C., Jr.
通讯作者：
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