Expression patterns of corticotropin-releasing factor, arginine vasopressin, histidine decarboxylase, melanin-concentrating hormone, and orexin genes in the human hypothalamus.
Expression patterns of corticotropin-releasing factor, arginine vasopressin, histidine decarboxylase, melanin-concentrating hormone, and orexin genes in the human hypothalamus.
复制标题
DOI：
10.1002/cne.22480
复制
发表时间：
2010-11-15
影响因子：
2.5
通讯作者：
Watson, Stanley J.
中科院分区：
文献类型：
--
作者：
Krolewski, David M.;Medina, Adriana;Kerman, Ilan A.;Bernard, Rene;Burke, Sharon;Thompson, Robert C.;Bunney, William E., Jr.;Schatzberg, Alan F.;Myers, Richard M.;Akil, Huda;Jones, Edward G.;Watson, Stanley J.
下丘脑调节许多[W2]自主反应和行为。下丘脑中产生的神经活性物质促肾上腺皮质激素释放因子（CRF）、精氨酸加压素（AVP）、组氨酸脱羧酶（HDC）、黑色素浓集激素（MCH）和食欲素/下丘脑分泌素（ORX）介导这些过程的一个子集。虽然这些基因的表达模式在啮齿动物中已经得到了很好的研究，但对人类的了解却很少。我们将经典的组织学技术与原位杂交组织化学相结合，以产生2维和3维图像，并在视觉上对齐和量化人类下丘脑核中这些物质的基因表达。将下丘脑任意分为吻侧区、中间区和尾侧区。包含室旁核（PVN）的吻侧区域由CRF和AVP表达神经元的离散定位定义，而HDC、MCH和ORX mRNA表达神经元之间的不同关系描绘了中间和尾侧区域内的特定水平。定量mRNA信号强度测量显示，在PVN的任何吻尾侧水平，总体CRF或AVP表达无显著差异。HDC mRNA在乳头体前区（包括乳头体背内侧核、乳头体结节核和下丘脑背外侧区）表达最高。此外，MCH和ORX mRNA表达神经元表现出的杂交信号的总体强度在不同的中间和尾部下丘脑区域达到峰值。这些结果表明，人类下丘脑神经元参与调节HPA轴显示不同的神经化学模式，可能包括多个本地核。
The hypothalamus regulates numerous [W2]autonomic responses and behaviors. The neuroactive substances corticotropin-releasing factor (CRF), arginine-vasopressin (AVP), histidine decarboxylase (HDC), melanin-concentrating hormone (MCH), and orexin/hypocretins (ORX) produced in the hypothalamus mediate a subset of these processes. Although the expression patterns of these genes have been well studied in rodents, less is known about them in humans. We combined classical histological techniques with in situ hybridization histochemistry to produce both 2 and 3-dimensional images and to visually align and quantify expression of the genes for these substances in nuclei of the human hypothalamus. The hypothalamus was arbitrarily divided into rostral, intermediate and caudal regions. The rostral region, containing the paraventricular nucleus (PVN), was defined by discrete localization of CRF and AVP expressing neurons, whereas distinct relationships between HDC, MCH, and ORX mRNA expressing neurons delineated specific levels within the intermediate and caudal regions. Quantitative mRNA signal intensity measurements revealed no significant differences in overall CRF or AVP expression at any rostro-caudal level of the PVN. HDC mRNA expression was highest at the level of the premammillary areawhich included the dorsomedial and tuberomammillary nuclei as well as the dorsolateral hypothalamic area. In addition, the overall intensity of hybridization signal exhibited by both MCH and ORX mRNA expressing neurons peaked in distinct intermediate and caudal hypothalamic regions. These results suggest that human hypothalamic neurons involved in the regulation of the HPA axis display distinct neurochemical patterns that may encompass multiple local nuclei.
登录
查看更多内容
影响因子：
2.5
作者：
Kerman, Ilan A.;Bernard, Rene;Watson, Stanley J.
通讯作者：
Watson, Stanley J.
影响因子：
3.2
作者：
Kennedy, AR;Todd, JF;Bloom, SR
通讯作者：
Bloom, SR
DOI：
10.1007/bf00309845
发表时间：
1987-01-01
期刊：
ANATOMY AND EMBRYOLOGY
影响因子：
--
作者：
BRAAK, H;BRAAK, E
通讯作者：
BRAAK, E
影响因子：
2.5
作者：
CASTREN, E;PANULA, P
通讯作者：
PANULA, P
DOI：
10.1073/pnas.181330998
发表时间：
2001-08-14
影响因子：
11.1
作者：
Huang, ZL;Qu, WM;Hayaishi, O
通讯作者：
Hayaishi, O