Mussel-inspired functionalization of electrospun scaffolds with polydopamine-assisted immobilization of mesenchymal stem cells-derived small extracellular vesicles for enhanced bone regeneration.
Mussel-inspired functionalization of electrospun scaffolds with polydopamine-assisted immobilization of mesenchymal stem cells-derived small extracellular vesicles for enhanced bone regeneration.
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受贻贝启发的电纺支架功能化，通过聚多巴胺辅助固定间充质干细胞衍生的小细胞外囊泡，以增强骨再生。
DOI：
10.1016/j.ijpharm.2021.121136
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发表时间：
2021-09
影响因子：
5.8
通讯作者：
Li Zubing
中科院分区：
文献类型：
--
作者：
Xing Xin;Han Shuang;Ni Yifeng;Cheng Gu;Cheng Yuet;Ni Xiaoqi;Deng Yunfan;Li Zhi;Li Zubing
间充质干细胞来源的细胞外小泡（MSCs-sEV）作为一种无细胞的骨组织再生策略显示出良好的前景。在此，采用贻贝固定化技术，以聚多巴胺（pDA）为辅助，制备了负载MSCs-sEV的静电纺丝丝素/聚己内酯（SF/PCL）生物支架。这种pDA修饰赋予所制备的支架高负载效率和sEV的持续释放曲线。体外细胞实验表明，负载MSCs-sEV的复合支架能促进前成骨细胞和内皮细胞的粘附和铺展，促进成骨分化和血管生成;体内实验表明，功能化的静电纺丝复合支架能促进大鼠颅骨缺损模型的骨再生。结果表明，开发的MSCs-sEV锚定PDA改性SF/PCL静电纺丝支架具有很高的应用潜力，在骨组织工程，由于其强大的促血管生成和成骨能力，无细胞生物活性，和成本效益。
Mesenchymal stem cells-derived small extracellular vesicles (MSCs-sEV) have shown promising prospects as a cell-free strategy for bone tissue regeneration. Here, a bioactive MSCs-sEV-loaded electrospun silk fibroin/poly(ε-caprolactone) (SF/PCL) scaffold was synthesized via a mussel-inspired immobilization strategy assisted by polydopamine (pDA). This pDA modification endowed the as-prepared scaffold with high loading efficiency and sustained release profile of sEV. In addition, the fabricated composite scaffold exhibited good physiochemical, mechanical, and biocompatible properties.In vitrocellular experiments indicated that the MSCs-sEV-loaded composite scaffold promoted the adhesion and spreading of preosteoblast and endothelial cells, as well as enhanced osteogenic differentiation and angiogenic activity.In vivoexperiments showed that the functionalized electrospun scaffolds promoted bone regeneration in a rat calvarial bone defect model. Results suggest that the developed MSCs-sEV-anchored pDA-modified SF/PCL electrospun scaffolds possess high application potential in bone tissue engineering owing to their powerful pro-angiogenic and -osteogenic capacities, cell-free bioactivity, and cost effectiveness.
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影响因子：
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DOI：
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发表时间：
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