Chlorogenic Acid Ameliorates Experimental Colitis by Promoting Growth of Akkermansia in Mice.
Chlorogenic Acid Ameliorates Experimental Colitis by Promoting Growth of Akkermansia in Mice.
复制标题
绿原酸通过促进小鼠阿克曼氏菌的生长来改善实验性结肠炎
作者：
Zhang Z;Wu X;Cao S;Cromie M;Shen Y;Feng Y;Yang H;Li L
绿原酸(CHA)是人类饮食中含量最丰富的多酚化合物之一，具有抗炎活性。本研究旨在探讨CHA对溃疡性结肠炎(UC)患者肠道微生物区系的影响。用2.5%葡聚糖硫酸钠(DSS)诱导C57BL/6小鼠结肠炎，对照组和饲料中添加CHA(1 MM)。观察组织病理学变化及炎症反应。粪便标本进行16S rRNA基因测序分析。CHA可减轻DSS诱导的结肠炎的几个效应，包括减轻体重、增加疾病活动指数和改善粘膜损伤。此外，CHA还可通过抑制活化的NF-γB信号通路，显著抑制F4/80+巨噬细胞、CD3+T细胞和CD177+中性粒细胞的分泌和F4/80+巨噬细胞、CD_3~+T细胞、CD_(177)+中性粒细胞的浸润，从而抑制干扰素α、肿瘤坏死因子κ和IL-6的分泌。此外，CHA还降低了细菌和拟杆菌的比例。CHA还促进了DSS处理小鼠粪便微生物区系多样性的减少。有趣的是，CHA治疗显著增加了粘液降解菌Akkermansia在结肠炎小鼠中的比例。CHA作为肠道微生物群落结构的修饰者，导致较低的肠道和全身炎症，并改善了DSS诱导的结肠炎的病程，这与Akkermansia的比例增加有关。
Chlorogenic acid (ChA)—one of the most abundant polyphenol compounds in the human diet—exerts anti-inflammatory activities. The aim of this study was to investigate the effect of ChA on gut microbiota in ulcerative colitis (UC). Colitis was induced by 2.5% dextran sulfate sodium (DSS) in C57BL/6 mice, which were on a control diet or diet with ChA (1 mM). The histopathological changes and inflammation were evaluated. Fecal samples were analyzed by 16S rRNA gene sequencing. ChA attenuated several effects of DSS-induced colitis, including weight loss, increased disease activity index, and improved mucosal damage. Moreover, ChA could significantly suppress the secretion of IFNγ, TNFα, and IL-6 and the colonic infiltration of F4/80+ macrophages, CD3+ T cells, and CD177+ neutrophils via inhibition of the active NF-κB signaling pathway. In addition, ChA decreased the proportion of Firmicutes and Bacteroidetes. ChA also enhanced a reduction in fecal microbiota diversity in DSS treated mice. Interestingly, ChA treatment markedly increased the proportion of the mucin-degrading bacterium Akkermansia in colitis mice. ChA acted as the intestine-modifying gut microbial community structure, resulting in a lower intestinal and systemic inflammation and also improving the course of the DSS-induced colitis, which is associated with a proportional increase in Akkermansia.
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影响因子：
--
作者：
Dueñas M;Muñoz-González I;Cueva C;Jiménez-Girón A;Sánchez-Patán F;Santos-Buelga C;Moreno-Arribas MV;Bartolomé B
通讯作者：
Bartolomé B
DOI：
10.1073/pnas.1219451110
发表时间：
2013-05-28
影响因子：
11.1
作者：
Everard, Amandine;Belzer, Clara;Cani, Patrice D.
通讯作者：
Cani, Patrice D.
影响因子：
4.3
作者：
Dai, Cong;Zheng, Chang-Qing;Jiang, Min
通讯作者：
Jiang, Min
影响因子：
24.5
作者：
Anhe, Fernando F.;Roy, Denis;Marette, Andre
通讯作者：
Marette, Andre
DOI：
10.1016/j.bpg.2013.03.005
发表时间：
2013-02-01
影响因子：
3.2
作者：
Berry, David;Reinisch, Walter
通讯作者：
Reinisch, Walter