Media Matters, Examining Historical and Modern Streptococcus pneumoniae Growth Media and the Experiments They Affect.
Media Matters, Examining Historical and Modern Streptococcus pneumoniae Growth Media and the Experiments They Affect.
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DOI：
10.3389/fcimb.2021.613623
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发表时间：
2021
影响因子：
5.7
通讯作者：
Johnson MDL
中科院分区：
文献类型：
--
作者：
Sanchez-Rosario Y;Johnson MDL
虽然一些细菌可以在矿物质和盐中繁衍生息，但许多细菌需要大量的营养和特定的化学物质才能生存到可以观察和研究的程度。尽管研究人员曾经通过煮沸并提炼动物的肉和骨头来获得促进细菌生长的培养基，但我们现在拥有大量的配方和制造商来提供历史、改良和现代培养基的脱水风味。媒体的目的已经从简单的隔离演变为更加谨慎的研究。然而，在某些情况下，为帮助微生物的代谢、营养或物理特性而配制的培养基可能并不最适合研究作为宿主相互作用的函数的病原体行为或恢复力。虽然对肺炎链球菌中的少数这些培养基进行了比较研究，但本综述重点描述了常见复合物（Todd Hewitt 和 M17）、半限定（Adams 和 Roe）和限定肺炎球菌培养基（RPMI 和 Van de Rijn 和 Kessler）的历史和现代组成、培养/生长增强发现/所需的关键成分，以及这些不同培养基对细菌表型和实验结果的影响。虽然许多研究人员找到了对他们选择的细菌进行生长和实验的最佳条件，但一些研究人员使用特定培养基的原因充其量是没有讨论的，而在最坏的情况下是任意的。因此，本次综述的目的是强调肺炎球菌培养基的差异，以鼓励研究人员质疑他们使用特定培养基的决定，讨论配方并解释他们的推理。
While some bacteria can thrive for generations in minerals and salts, many require lavish nutrition and specific chemicals to survive to the point where they can be observed and researched. Although researchers once boiled and rendered animal flesh and bones to obtain a media that facilitated bacterial growth, we now have a plethora of formulations and manufacturers to provide dehydrated flavors of historical, modified, and modern media. The purpose of media has evolved from simple isolation to more measured study. However, in some instances, media formulated to aid the metabolic, nutritional, or physical properties of microbes may not be best suited for studying pathogen behavior or resilience as a function of host interactions. While there have been comparative studies on handfuls of these media in Streptococcus pneumoniae, this review focuses on describing both the historical and modern composition of common complex (Todd Hewitt and M17), semi-defined (Adams and Roe), and defined pneumococcal media (RPMI and Van de Rijn and Kessler), key components discovered/needed for cultivation/growth enhancement, and effects these different media have on bacterial phenotypes and experimental outcomes. While many researchers find the best conditions to grow and experiment on their bacteria of choice, the reasons for some researchers to use a specific medium is at best, not discussed, and at worst, arbitrary. As such, the goal of this review is to highlight the differences in pneumococcal media to encourage investigators to challenge their decisions on why they use a given medium, discuss the recipe, and explain their reasoning.
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影响因子：
6.4
作者：
ELLNER, PD;STOESSEL, CJ
通讯作者：
STOESSEL, CJ
影响因子：
3.6
作者：
Brown, JS;Gilliland, SM;Holden, DW
通讯作者：
Holden, DW
影响因子：
15.3
作者：
DAVIS, BD;DUBOS, RJ
通讯作者：
DUBOS, RJ
影响因子：
3.7
作者：
Carvalho SM;Kuipers OP;Neves AR
通讯作者：
Neves AR
研究肺炎球菌类型的诱导物质转化的化学性质：通过从III型肺炎球菌分离出的脱氧核糖核酸分数诱导转化。
DOI：
10.1084/jem.79.2.137
发表时间：
1944-02-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Avery OT;Macleod CM;McCarty M
通讯作者：
McCarty M