Adaptation of a simple dipstick test for detection of Vibrio cholerae O1 and O139 in environmental water.
Adaptation of a simple dipstick test for detection of Vibrio cholerae O1 and O139 in environmental water.
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DOI：
10.3389/fmicb.2013.00320
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发表时间：
2013
影响因子：
5.2
通讯作者：
Sack DA
中科院分区：
文献类型：
--
作者：
Chakraborty S;Alam M;Scobie HM;Sack DA
环境中霍乱弧菌的存在是了解霍乱流行病学的关键。实验室确认水中霍乱弧菌的金标准是培养方法，但这需要实验室基础设施。一种简单、廉价、可广泛应用的快速诊断试验将有助于确认环境水样中的霍乱弧菌。在这里，我们评估了试纸测试，以检测加标样品和现场条件下的环境水样中的霍乱弧菌O1和O139。当环境水样在碱性蛋白胨水中室温孵育24 h时，使用试纸测试可以检测到添加<10 CFU的样品。与培养相比，该测试对环境样品的敏感性为89%，特异性为100%。
The presence of Vibrio cholerae in the environment is key to understanding the epidemiology of cholera. The gold standard for laboratory confirmation of V. cholerae from water is a culture method, but this requires laboratory infrastructure. A rapid diagnostic test that is simple, inexpensive, and can be deployed widely would be useful for confirming V. cholerae in samples of environmental water. Here, we evaluated a dipstick test to detect V. cholerae O1 and O139 from environmental water samples in spiked samples and under field conditions. When environmental water samples were incubated in alkaline peptone water for 24 h at room temperature, samples spiked with <10 CFU could be detected using the dipstick test. When compared to culture, the test was 89% sensitive and 100% specific with environmental samples.
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影响因子：
9.4
作者：
Bhuiyan, NA;Qadri, F;Nair, GB
通讯作者：
Nair, GB
影响因子：
3.7
作者：
Rosewell, Alexander;Addy, Benita;MacIntyre, C. Raina
通讯作者：
MacIntyre, C. Raina
影响因子：
3.7
作者：
Wang, XY;Ansaruzzaman, M;Fournier, JM
通讯作者：
Fournier, JM
影响因子：
3.3
作者：
Harris, Julie R.;Cavallaro, Elizabeth C.;Mintz, Eric D.
通讯作者：
Mintz, Eric D.
DOI：
10.1128/cdli.10.3.476-478.2003
发表时间：
2003-05-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
--
作者：
Nato, F;Boutonnier, A;Chanteau, S
通讯作者：
Chanteau, S