QTL Mapping of Seed Glucosinolate Content Responsible for Environment in Brassica napus.
QTL Mapping of Seed Glucosinolate Content Responsible for Environment in Brassica napus.
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甘蓝型油菜种子芥子油苷含量对环境负责的QTL定位
DOI：
10.3389/fpls.2018.00891
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发表时间：
2018
影响因子：
5.6
通讯作者：
Qian W
中科院分区：
文献类型：
--
作者：
He Y;Fu Y;Hu D;Wei D;Qian W
硫代葡萄糖苷（GSL）是一类重要的次生代谢产物。甘蓝型油菜种子GSL含量在很大程度上受环境调节。而B种子GSL含量的遗传控制主要受环境因素的影响。人们对油菜的了解很少。本研究以冬性和半冬性油菜杂交的双单倍体（DH）群体为材料，在德国和中国两个不同的生态环境中种植，研究了生态环境对油菜种子GSL的影响，并分析了影响种子GSL的环境数量性状位点（QTL）。对DH群体中各株系的GSL含量进行了生态环境间偏差值（GSLE）的计算，并检测了GSLE的QTL。DH群体的GSL介于−46.90 ~ 36.13 μmol g−1粗粉之间，表明油菜种子GSL具有显著的生态环境效应。在A04、A06和A09染色体上共检测到4个GSLE QTL，解释表型变异的4.70 ~ 9.93%。通过对不同生态环境下种子GSL含量QTL的比较，发现3个GSL QTL在A02（37.6 ~ 45.4cM）、A04（0 ~ 17.2cM）和A09（67.0 ~ 98.6cM）上的效应在德国和中国生态环境下差异显著（P < 0.01），表明这些基因座对种子GSL含量具有环境敏感性。此外，本研究还对植物重要的环境适应性状开花时间（FT）进行了研究。对GSLE、GSL和FT的QTL分析表明，A02、A04和A09染色体上的3个区域不仅在德国和中国之间的QTL效应存在显著差异，而且与GSLE和FT的QTL区间共定位。结果表明，大多数GSL位点在不同的生态环境条件下都能影响GSL的积累，而A02、A04和A09上的3个QTL区间可能对种子GSL含量的生态环境敏感。
Glucosinolates (GSLs) are a major class of secondary metabolites. The content of seed GSL is largely regulated by environments in rapeseed (Brassica napus). However, the genetic control of seed GSL content responsible for environment in B. napus has been poorly understood. In the current study, a doubled haploid (DH) population from a cross between winter and semi-winter lines of rapeseed was grown in two distinct eco-environments, Germany and China, to evaluate the eco-environment effect and dissect the quantitative trait loci (QTL) responsible for environment for seed GSL in rapeseed. The deviation value of GSL content between eco-environments (GSLE) was calculated for each line in the DH population and the QTLs for GSLE were detected. GSLE ranged from −46.90 to 36.13 μmol g−1 meal in the DH population, suggesting the prominent eco-environmental effects for seed GSL in rapeseed. Four QTLs for GSLE were identified on chromosomes A04, A06, and A09 explaining 4.70∼9.93% of the phenotypic variation. Comparison of QTLs of seed GSL content between different eco-environments found three QTLs for GSL on A02 from 37.6 to 45.4 cM, A04 from 0 to 17.2 cM, and A09 from 67.0 to 98.6 cM exhibited significant difference of QTL effect between the German and Chinese eco-environments (P < 0.01), indicating the environment sensibility of these loci on seed GSL content. Moreover, flowering time (FT), an important environment adaptation trait in plant, was also investigated in this study. Comparative QTL analysis among GSLE, GSL, and FT revealed that three regions on chromosomes A02, A04, and A09 not only exhibited significant differences in QTL effect between Germany and China, but also co-located with the QTL intervals of GSLE and FT. Our results revealed that most of the GSL loci can influence GSL accumulation under different eco-environments, whereas the three QTL intervals on A02, A04, and A09 might be sensitive to the eco-environments for seed GSL content.
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DOI：
10.1093/dnares/dsu002
发表时间：
2014-08
期刊：
DNA research : an international journal for rapid publication of reports on genes and genomes
影响因子：
--
作者：
Li F;Chen B;Xu K;Wu J;Song W;Bancroft I;Harper AL;Trick M;Liu S;Gao G;Wang N;Yan G;Qiao J;Li J;Li H;Xiao X;Zhang T;Wu X
通讯作者：
Wu X
影响因子：
7.4
作者：
Mendez-Vigo, Belen;Xavier Pico, F.;Alonso-Blanco, Carlos
通讯作者：
Alonso-Blanco, Carlos
影响因子：
3.1
作者：
Sharpe, AG;Lydiate, DJ
通讯作者：
Lydiate, DJ
影响因子：
3.1
作者：
Howell, PM;Sharpe, AG;Lydiate, DJ
通讯作者：
Lydiate, DJ
影响因子：
4.8
作者：
Inouye, David W.
通讯作者：
Inouye, David W.