Omics of an Enigmatic Marine Amoeba Uncovers Unprecedented Gene Trafficking from Giant Viruses and Provides Insights into Its Complex Life Cycle.
Omics of an Enigmatic Marine Amoeba Uncovers Unprecedented Gene Trafficking from Giant Viruses and Provides Insights into Its Complex Life Cycle.
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DOI：
10.3390/microbiolres14020047
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发表时间：
2023-06
影响因子：
1.5
通讯作者：
Udu I
中科院分区：
文献类型：
--
作者：
Tekle YI;Tran H;Wang F;Singla M;Udu I
变形虫包括多种生态、行为和形态的谱系。它们被认为包含了所有生物中基因组大小最大的成员，然而对该群体的基因组研究是有限的。Trichosphaerium是一种多态、多核、具有复杂生命周期的海洋变形虫，一个多世纪以来一直困扰着专家们。为了探索阿米巴原虫的基因组多样性和研究在阿米巴原虫中观察到的非凡行为，我们使用综合组学方法研究了这种神秘的海洋阿米巴原虫。包括单细胞转录组学和细胞学数据在内的组学数据表明，Trichosphaerium sp.拥有完整的减数分裂工具箱基因。这些基因在变形虫的生命阶段包括中、大细胞中都有表达。Trichosphaerium sp.的生命周期包括通过二元分裂和巨细胞多次分裂的无性过程，以及涉及有性生殖和多倍体化的基因的性样过程。这些发现与之前报道的这种变形虫的生命周期形成鲜明对比。尽管在Trichosphaerium中观察到极端的形态可塑性，但我们的基因组数据表明，种群保持着物种水平的基因组内变异。Trichosphaerium的基因组草图表明，细菌和巨型病毒的横向基因转移（LGT）增加。在阿米巴虫和真核微生物中，Trichosphaerium的基因运输是最高的。
Amoebozoa include lineages of diverse ecology, behavior, and morphology. They are assumed to encompass members with the largest genome sizes of all living things, yet genomic studies in the group are limited. Trichosphaerium, a polymorphic, multinucleate, marine amoeba with a complicated life cycle, has puzzled experts for over a century. In an effort to explore the genomic diversity and investigate extraordinary behavior observed among the Amoebozoa, we used integrated omics approaches to study this enigmatic marine amoeba. Omics data, including single-cell transcriptomics and cytological data, demonstrate that Trichosphaerium sp. possesses the complete meiosis toolkit genes. These genes are expressed in life stages of the amoeba including medium and large cells. The life cycle of Trichosphaerium sp. involves asexual processes via binary fission and multiple fragmentation of giant cells, as well as sexual-like processes involving genes implicated in sexual reproduction and polyploidization. These findings are in stark contrast to a life cycle previously reported for this amoeba. Despite the extreme morphological plasticity observed in Trichosphaerium, our genomic data showed that populations maintain a species-level intragenomic variation. A draft genome of Trichosphaerium indicates elevated lateral gene transfer (LGT) from bacteria and giant viruses. Gene trafficking in Trichosphaerium is the highest within Amoebozoa and among the highest in microbial eukaryotes.
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影响因子：
10.7
作者：
Kang S;Tice AK;Spiegel FW;Silberman JD;Pánek T;Cepicka I;Kostka M;Kosakyan A;Alcântara DMC;Roger AJ;Shadwick LL;Smirnov A;Kudryavtsev A;Lahr DJG;Brown MW
通讯作者：
Brown MW
影响因子：
64.8
作者：
Loftus, B;Anderson, I;Hall, N
通讯作者：
Hall, N
DOI：
10.1073/pnas.1714590114
发表时间：
2017-12-19
影响因子：
11.1
作者：
Cornelis, Guillaume;Funk, Mathis;Heidmann, Thierry
通讯作者：
Heidmann, Thierry
DOI：
10.1099/00207713-52-5-1893
发表时间：
2002-09-01
影响因子：
2.8
作者：
Katz, LA
通讯作者：
Katz, LA
对真核生物的分类，命名法和多样性进行修订。
DOI：
10.1111/jeu.12691
发表时间：
2019-01
期刊：
The Journal of eukaryotic microbiology
影响因子：
--
作者：
Adl SM;Bass D;Lane CE;Lukeš J;Schoch CL;Smirnov A;Agatha S;Berney C;Brown MW;Burki F;Cárdenas P;Čepička I;Chistyakova L;Del Campo J;Dunthorn M;Edvardsen B;Eglit Y;Guillou L;Hampl V;Heiss AA;Hoppenrath M;James TY;Karnkowska A;Karpov S;Kim E;Kolisko M;Kudryavtsev A;Lahr DJG;Lara E;Le Gall L;Lynn DH;Mann DG;Massana R;Mitchell EAD;Morrow C;Park JS;Pawlowski JW;Powell MJ;Richter DJ;Rueckert S;Shadwick L;Shimano S;Spiegel FW;Torruella G;Youssef N;Zlatogursky V;Zhang Q
通讯作者：
Zhang Q