Correlation of IgE/IgG4 milk epitopes and affinity of milk-specific IgE antibodies with different phenotypes of clinical milk allergy.
Correlation of IgE/IgG4 milk epitopes and affinity of milk-specific IgE antibodies with different phenotypes of clinical milk allergy.
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DOI：
10.1016/j.jaci.2009.12.017
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发表时间：
2010-03
影响因子：
14.2
通讯作者：
Sampson, Hugh A.
中科院分区：
文献类型：
--
作者：
Wang, Julie;Lin, Jing;Bardina, Ludmilla;Goldis, Marina;Nowak-Wegrzyn, Anna;Shreffler, Wayne G.;Sampson, Hugh A.
使用肽微阵列进行大规模表位作图的结果已被证明与牛奶过敏的临床特征相关。我们试图评估牛奶过敏不同临床表型中的 IgE 和 IgG4 表位多样性和 IgE 亲和力，并确定可预测牛奶过敏临床结果的信息性表位。一项更大规模的研究招募了 41 名受试者，该研究旨在研究牛奶过敏个体摄入热变性牛奶蛋白的影响。通过食物挑战，受试者被描述为对所有形式的牛奶有临床反应（n = 17）、对加热牛奶（HM）产品具有耐受性（n = 16）或随着年龄的增长不再过敏（n = 8）。十一名非牛奶过敏的健康志愿者作为对照。使用先前发布的方案进行肽微阵列。与那些不再过敏的人相比，牛奶过敏的受试者的表位多样性有所增加。 HM 耐受受试者的 IgE 结合模式与那些不再过敏的受试者相似，但 IgG4 结合模式与过敏组更相似。与更多数量的 IgE 肽结合与攻击过程中更严重的过敏反应相关。 IgG4 肽与牛奶过敏的临床特征之间没有关联。使用竞争性肽微阵列测定，过敏患者表现出高亲和力和低亲和力 IgE 结合的组合，而 HM 耐受受试者和那些已经不再对牛奶过敏的受试者主要具有低亲和力结合。通过肽微阵列测定，更大的 IgE 表位多样性和更高的亲和力与临床表型和牛奶过敏的严重程度相关。
Results from large-scale epitope mapping using peptide microarray have been shown to correlate with clinical features of milk allergy. We sought to assess IgE and IgG4 epitope diversity and IgE affinity in different clinical phenotypes of milk allergy and identify informative epitopes that may be predictive of clinical outcomes of milk allergy. Forty-one subjects were recruited from a larger study on the effects of ingesting heat-denatured milk proteins in milk-allergic individuals. Using food challenges, subjects were characterized as clinically reactive to all forms of milk (n = 17), tolerant to heated milk (HM) products (n = 16), or outgrown their milk allergy (n = 8). Eleven non-milk allergic, healthy volunteers served as controls. Peptide microarray was performed using the previously published protocol. Milk allergic subjects had increased epitope diversity as compared to those who outgrew their allergy. HM tolerant subjects had IgE binding patterns similar to those who had outgrown their allergy, but IgG4 binding patterns that were more similar to the allergic group. Binding to higher numbers of IgE peptides was associated with more severe allergic reactions during challenge. There was no association between IgG4 peptides and clinical features of milk allergy. Using a competitive peptide microarray assay, allergic patients demonstrated a combination of high and low affinity IgE binding whereas HM tolerant subjects and those who had outgrown their milk allergy had primarily low affinity binding. Greater IgE epitope diversity and higher affinity as determined by peptide microarray were associated with clinical phenotypes and severity of milk allergy.
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影响因子：
6.1
作者：
Mita, H;Yasueda, H;Akiyama, K
通讯作者：
Akiyama, K
影响因子：
4.4
作者：
El-Khouly, Fatima;Lewis, Stella Anne;Hourihane, Jonathan O'Brien
通讯作者：
Hourihane, Jonathan O'Brien
影响因子：
6.1
作者：
Lewis, SA;Grimshaw, KEC;Hourihane, JOB
通讯作者：
Hourihane, JOB
影响因子：
14.2
作者：
Flinterman, Annebeth E.;Knol, Edward F.;Shreffler, Wayne G.
通讯作者：
Shreffler, Wayne G.
影响因子：
14.2
作者：
Skripak, Justin M.;Matsui, Elizabeth C.;Wood, Robert A.
通讯作者：
Wood, Robert A.