Microbubble-mediated ultrasound enhances the lethal effect of gentamicin on planktonic Escherichia coli.
Microbubble-mediated ultrasound enhances the lethal effect of gentamicin on planktonic Escherichia coli.
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微生物介导的超声可以增强庆大霉素对浮游大肠杆菌的致命作用。
DOI：
10.1155/2014/142168
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发表时间：
2014
影响因子：
--
通讯作者：
Yan SG
中科院分区：
文献类型：
--
作者：
Zhu HX;Cai XZ;Shi ZL;Hu B;Yan SG
先前的研究发现，低强度超声增强庆大霉素对大肠杆菌的致死作用。杆菌我们的目的是进一步研究微泡介导的低强度超声是否可以进一步增强庆大霉素的抗菌效果。对E.大肠杆菌（ATCC 25922）的细胞被分配到四种不同的干预：对照（GCON）、仅微泡（GMB）、仅超声（GUS）和微泡介导的超声（GMUS）。施加100 mW/cm 2（平均强度）和46.5 KHz的超声波，其没有杀菌活性。培养12 h后，采用平板计数法测定细菌数量，透射电镜观察细菌的显微形态。结果表明，大肠杆菌活菌数与对照组相比，差异有统计学意义（P < 0. 05）。大肠杆菌在GMUS中的表达量比GUS低1.01 ~ 1.42 log 10 CFU/mL（P < 0.01）。庆大霉素对大肠杆菌的最低抑菌浓度（MIC）为0.001。在GMUS组和GUS组中，大肠杆菌的表达量为1 μg/mL，低于GCON组和GMB组（2 μg/mL）。透射电子显微镜（TEM）图像显示更多的破坏和更厚的细菌细胞膜在GMUS比其他组。其原因可能是声空化作用导致细胞膜对庆大霉素的通透性增加。
Previous research has found that low-intensity ultrasound enhanced the lethal effect of gentamicin on planktonic E. coli. We aimed to further investigate whether microbubble-mediated low-intensity ultrasound could further enhance the antimicrobial efficacy of gentamicin. The planktonic E. coli (ATCC 25922) was distributed to four different interventions: control (GCON), microbubble only (GMB), ultrasound only (GUS), and microbubble-mediated ultrasound (GMUS). Ultrasound was applied with 100 mW/cm2 (average intensity) and 46.5 KHz, which presented no bactericidal activity. After 12 h, plate counting was used to estimate the number of bacteria, and bacterial micromorphology was observed with transmission electron microscope. The results showed that the viable counts of E. coli in GMUS were decreased by 1.01 to 1.42 log10 CFU/mL compared with GUS (P < 0.01). The minimal inhibitory concentration (MIC) of gentamicin against E. coli was 1 μg/mL in the GMUS and GUS groups, lower than that in the GCON and GMB groups (2 μg/mL). Transmission electron microscopy (TEM) images exhibited more destruction and higher thickness of bacterial cell membranes in the GMUS than those in other groups. The reason might be the increased permeability of cell membranes for gentamicin caused by acoustic cavitation.
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影响因子：
2.9
作者：
Williams, RG;Pitt, WG
通讯作者：
Pitt, WG
间歇性瓦特级超声波促进万古霉素从治疗性丙烯酸骨水泥中释放。
DOI：
10.1002/jbm.b.31288
发表时间：
2009-07-01
影响因子：
3.4
作者：
Cai, Xun-Zi;Chen, Xian-Zhen;Xu, Jia
通讯作者：
Xu, Jia
影响因子：
1.2
作者：
Rediske, AM;Hymas, WC;Pitt, WG
通讯作者：
Pitt, WG
影响因子：
1.2
作者：
Runyan, Christopher M.;Carmen, John C.;Pitt, William G.
通讯作者：
Pitt, William G.
影响因子：
8.4
作者：
Ikeda-Dantsuji, Yurika;Feril, Loreto B., Jr.;Maruyama, Kazuo
通讯作者：
Maruyama, Kazuo