Phylogenetic analysis of Bolivian bat trypanosomes of the subgenus schizotrypanum based on cytochrome B sequence and minicircle analyses.
Phylogenetic analysis of Bolivian bat trypanosomes of the subgenus schizotrypanum based on cytochrome B sequence and minicircle analyses.
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DOI：
10.1371/journal.pone.0036578
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Solari A
中科院分区：
文献类型：
--
作者：
García L;Ortiz S;Osorio G;Torrico MC;Torrico F;Solari A
本研究的目的是建立玻利维亚卡罗莱亚蝙蝠血液样本中存在的锥虫的系统发育关系。从玻利维亚查帕雷省3个亚马孙地区的115只佩皮卡罗莱亚（Phyllostomidae）蝙蝠中分离出18个克隆种群，并利用粗大罗氏虫和感染三角虫（克氏马伦克莱锥虫）或血液培养（dionisit锥虫）进行异种诊断研究。利用编码细胞色素b的大圆环核苷酸序列和小圆环超变区分子大小对扩增的DNA进行分析。其中10份为克氏滨海锥虫，8份为戴奥尼氏锥虫。这两个物种在微环扩增区以及用于比较的克氏锥虫和兰氏锥虫方面具有不同的分子大小特征。我们得出结论，在这些样本中存在两种蝙蝠锥虫，可以通过本研究中使用的方法清楚地识别出来。讨论了这些锥虫在亚马逊蝙蝠中的存在。
The aim of this study was to establish the phylogenetic relationships of trypanosomes present in blood samples of Bolivian Carollia bats. Eighteen cloned stocks were isolated from 115 bats belonging to Carollia perspicillata (Phyllostomidae) from three Amazonian areas of the Chapare Province of Bolivia and studied by xenodiagnosis using the vectors Rhodnius robustus and Triatoma infestans (Trypanosoma cruzi marenkellei) or haemoculture (Trypanosoma dionisii). The PCR DNA amplified was analyzed by nucleotide sequences of maxicircles encoding cytochrome b and by means of the molecular size of hyper variable regions of minicircles. Ten samples were classified as Trypanosoma cruzi marinkellei and 8 samples as Trypanosoma dionisii. The two species have a different molecular size profile with respect to the amplified regions of minicircles and also with respect to Trypanosoma cruzi and Trypanosoma rangeli used for comparative purpose. We conclude the presence of two species of bat trypanosomes in these samples, which can clearly be identified by the methods used in this study. The presence of these trypanosomes in Amazonian bats is discussed.
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影响因子：
4
作者：
Cavazzana, Manzelio, Jr.;Marcili, Arlei;Teixeira, Marta M. G.
通讯作者：
Teixeira, Marta M. G.
DOI：
10.4269/ajtmh.1978.27.483
发表时间：
1978-01-01
影响因子：
3.3
作者：
BAKER, JR;MILES, MA;BARRETT, TV
通讯作者：
BARRETT, TV
影响因子：
3.2
作者：
Adolfo Vallejo, Gustavo;Guhl, Felipe;Grisard, Edmundo Carlos
通讯作者：
Grisard, Edmundo Carlos
影响因子：
2.6
作者：
Lisboa, Cristiane Varella;Pinho, Ana Paula;Jansen, Ana Maria
通讯作者：
Jansen, Ana Maria
DOI：
10.1080/00034983.1982.11687517
发表时间：
1982-01-01
影响因子：
--
作者：
MARINKELLE, CJ
通讯作者：
MARINKELLE, CJ