Individual and Synergistic Antioxidant Effects of Dipeptides in In Vitro Antioxidant Evaluation Systems
Individual and Synergistic Antioxidant Effects of Dipeptides in In Vitro Antioxidant Evaluation Systems
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体外抗氧化评价系统中二肽的单独和协同抗氧化作用
DOI：
10.1007/s10989-018-9684-y
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发表时间：
2019-03
影响因子：
2.5
通讯作者：
Zhang Ting
中科院分区：
文献类型：
--
作者：
Du Zhiyang;Liu Jingbo;Zhang Deju;Ding Long;Wang Yizhen;Tan Dawei;Zhang Ting
本研究旨在研究二肽在体外的个体和协同抗氧化作用。基于氨基酸抗氧化活性的研究，选择了 36 种二肽。他们所有的抗氧化效果均在体外抗氧化评估系统中进行了测试，包括 DPPH、ABTS、ORAC 和 FRAP 测定。根据这些二肽各自的抗氧化作用结果，选择其中一些二肽来研究它们在四种抗氧化测定中的协同抗氧化作用。 DPPH测定结果表明，含有Cys的二肽表现出自由基清除活性。协同抗氧化试验表明，在DPPH试验中，LC和CH、LC和CN、LC和CE、CH和CN、CH和CE、CN和CE具有显着的协同作用(p<0.01)。此外，ABTS测定结果表明，17种二肽具有ABTS自由基清除活性，并且均含有Cys、Trp和Tyr残基。他们的协同抗氧化试验表明，CR 和 CH、YR 和 YK、YR 和 YN、WK 和 IW 表现出高度显着的协同抗氧化作用（p<0.01）。 ORAC测定结果表明，22种二肽具有ORAC活性，且均含有1个或2个Cys、His、Met、Trp和Tyr残基。 CH与HL、WK与WR、YK与WR、LC与FM、FM与CH、WK与IW的协同效应均显着(p<<0.01)。最后，在 FRAP 测定中，六种二肽显示出铁降低抗氧化活性，并且它们都含有 Cys 残基。 HV和CN、CK和CN、CH和CN、CE和CN的协同抗氧化作用也显着(p<0.01)。这些发现表明二肽的单独和协同抗氧化作用与其组成氨基酸有关。这些结果可能有助于研究二肽的其他单独和协同抗氧化作用。
This study aimed to investigate the individual and synergistic antioxidant effects of dipeptides in vitro. Based on a study of amino acid antioxidant activities, 36 dipeptides were chosen. All their individual antioxidant effects were tested in in vitro antioxidant evaluation systems, including the DPPH, ABTS, ORAC, and FRAP assays. Based on the results of individual antioxidant effects of these dipeptides, some of them were chosen to study their synergistic antioxidant effects in the four antioxidant assays. The results of the DPPH assay showed that dipeptides containing Cys exhibited radical scavenging activity. The synergistic antioxidant assay indicated that LC and CH, LC and CN, LC and CE, CH and CN, CH and CE, and CN and CE have significant synergistic effects (p < 0.01) in the DPPH assay. Moreover, the results of the ABTS assay showed that 17 dipeptides have ABTS radical scavenging activities and that all of them contain Cys, Trp, and Tyr residues. Their synergistic antioxidant assay showed that CR and CH, YR and YK, YR and YN, and WK and IW showed highly significant synergistic antioxidant effects (p < 0.01). The results of the ORAC assay showed that 22 dipeptides possessed ORAC activities and that all of them have one or two Cys, His, Met, Trp, and Tyr residues. The synergistic effects of CH and HL, WK and WR, YK and WR, LC and FM, FM and CH, and WK and IW were significant (p < 0.01). Finally, in the FRAP assay, six dipeptides showed ferric reducing antioxidant activities and all of them contained the Cys residue. The synergistic antioxidant effects of HV and CN, CK and CN, CH and CN, and CE and CN were also significant (p < 0.01). These findings indicate that the individual and synergistic antioxidant effects of dipeptides are related to their constituent amino acids. These results may help study other individual and synergistic antioxidant effects of dipeptides.
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影响因子：
5.6
作者：
Chompoonuch Wiriyaphan;B. Chitsomboon;S. Roytrakul;Jirawat Yongsawadigul
通讯作者：
Chompoonuch Wiriyaphan;B. Chitsomboon;S. Roytrakul;Jirawat Yongsawadigul
影响因子：
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作者：
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影响因子：
2.3
作者：
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通讯作者：
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影响因子：
8.8
作者：
Becker, Eleonora Miquel;Ntouma, Georgia;Skibsted, Leif H.
通讯作者：
Skibsted, Leif H.
影响因子：
8.8
作者：
Liu, Jingbo;Jin, Yan;Chen, Feng
通讯作者：
Chen, Feng