Developmental transcriptome of Aplysia californica.
Developmental transcriptome of Aplysia californica.
复制标题
海兔的发育转录组。
DOI：
10.1002/jez.b.21383
复制
发表时间：
2011-03-15
影响因子：
2.2
通讯作者：
Moroz, Leonid L.
中科院分区：
文献类型：
--
作者：
Heyland, Andreas;Vue, Zer;Voolstra, Christian R.;Medina, Monica;Moroz, Leonid L.
发育过程中全基因组的转录变化为了解生长、分化和模式的机制提供了重要的见解。然而，这种大规模的发育研究仅限于蜕皮动物和脊索动物的少数代表。在这里，我们表征了海洋软体动物加州海兔的胚胎、幼虫和变态发育的转录组，并揭示了与生命史转变相关的新分子成分。具体来说，我们鉴定了 20 多种与早期发育和变态阶段相关的信号肽、假定的激素和转录因子，其中许多似乎是信号转导途径的进化保守元件。我们还描述了与生物矿化相关的基因——这是软体动物发育的关键过程。总之，我们的实验首次对软体动物发育中的基因表达进行了大规模调查，并补充了先前关于身体计划模式和幼虫和幼体结构形成的调节机制的研究。这项研究为海兔（特别是软体动物）的转录本和基因的进一步功能注释提供了资源。将海兔发育转录组与斑马鱼斑马鱼、果蝇、线虫秀丽隐杆线虫和其他软体动物研究中的类似研究进行比较表明，基因调控机制总体上存在高度差异的模式，这可能是这些生物体不同发育模式的结果。
Genome-wide transcriptional changes in development provide important insight into mechanisms underlying growth, differentiation, and patterning. However, such large-scale developmental studies have been limited to a few representatives of Ecdysozoans and Chordates. Here, we characterize transcriptomes of embryonic, larval, and metamorphic development in the marine mollusc Aplysia californica and reveal novel molecular components associated with life history transitions. Specifically, we identify more than 20 signal peptides, putative hormones, and transcription factors in association with early development and metamorphic stages—many of which seem to be evolutionarily conserved elements of signal transduction pathways. We also characterize genes related to biomineralization—a critical process of molluscan development. In summary, our experiment provides the first large-scale survey of gene expression in mollusc development, and complements previous studies on the regulatory mechanisms underlying body plan patterning and the formation of larval and juvenile structures. This study serves as a resource for further functional annotation of transcripts and genes in Aplysia, specifically and molluscs in general. A comparison of the Aplysia developmental transcriptome with similar studies in the zebra fish Danio rerio, the fruit fly Drosophila melanogaster, the nematode Caenorhabditis elegans, and other studies on molluscs suggests an overall highly divergent pattern of gene regulatory mechanisms that are likely a consequence of the different developmental modes of these organisms.
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影响因子：
3
作者：
He J;Dai X;Zhao X
通讯作者：
Zhao X
DOI：
10.1002/neu.480220905
发表时间：
1991-12-01
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
DIEFENBACH, TJ;KOEHNCKE, NK;GOLDBERG, JI
通讯作者：
GOLDBERG, JI
DOI：
10.1016/j.cbd.2010.03.003
发表时间：
2010-06
影响因子：
3
作者：
Fiedler, T. J.;Hudder, A.;Mckay, S. J.;Shivkumar, S.;Capo, T. R.;Schmale, M. C.;Walsh, P. J.
通讯作者：
Walsh, P. J.
影响因子：
3.8
作者：
ARCH, S
通讯作者：
ARCH, S
DOI：
10.1002/jez.b.21113
发表时间：
2006-11-15
影响因子：
2.2
作者：
Heyland, Andreas;Price, David A.;Moroz, Leonid L.
通讯作者：
Moroz, Leonid L.