A graphene aptasensor for biomarker detection in human serum.
A graphene aptasensor for biomarker detection in human serum.
复制标题
用于人血清中生物标志物检测的石墨烯适中。
DOI：
10.1016/j.electacta.2018.08.062
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发表时间：
2018-11-10
影响因子：
6.6
通讯作者：
Lin Q
中科院分区：
文献类型：
--
作者：
Wang X;Zhu Y;Olsen TR;Sun N;Zhang W;Pei R;Lin Q
本文提出了一种亲和石墨烯纳米传感器，用于检测未稀释和未脱盐的人血清中的生物标志物。亲和纳米传感器是一种场效应晶体管，其中石墨烯作为导电通道。石墨烯表面依次用聚合物聚乙二醇（PEG）和生物标记物特异性适体的纳米层官能化。适体能够特异性结合并捕获血清中未标记的生物标志物。捕获的生物标志物会引起石墨烯电导率的变化，该变化在最佳选择的离子强度的缓冲液中进行测量，以确定生物标志物的浓度。PEG层有效地拒绝血清中背景分子的非特异性吸附，同时仍然允许适体容易接近血清携带的生物标志物，并增加石墨烯表面上的有效德拜筛选长度。因此，适体-生物标志物结合灵敏地改变石墨烯电导率，从而以高灵敏度实现生物标志物的特异性和无标记检测，而不需要稀释或脱盐血清。实验结果表明，石墨烯纳米传感器能够特异性捕获人血清中50 pM-250 nM浓度范围内的人免疫球蛋白E（IgE），用作代表性生物标志物，分辨率为14.5 pM，检测限为47 pM。
This paper presents an affinity graphene nanosensor for detection of biomarkers in undiluted and non-desalted human serum. The affinity nanosensor is a field-effect transistor in which graphene serves as the conducting channel. The graphene surface is sequentially functionalized with a nanolayer of the polymer polyethylene glycol (PEG) and a biomarker-specific aptamer. The aptamer is able to specifically bind with and capture unlabeled biomarkers in serum. A captured biomarker induces a change in the electric conductivity of the graphene, which is measured in a buffer of optimally chosen ionic strength to determine the biomarker concentration. The PEG layer effectively rejects nonspecific adsorption of background molecules in serum while still allowing the aptamer to be readily accessible to serum-borne biomarkers and increases the effective Debye screening length on the graphene surface. Thus, the aptamer-biomarker binding sensitively changes the graphene conductivity, thereby achieving specific and label-free detection of biomarkers with high sensitivity and without the need to dilute or desalt the serum. Experimental results demonstrate that the graphene nanosensor is capable of specifically capturing human immunoglobulin E (IgE), used as a representative biomarker, in human serum in the concentration range of 50 pM–250 nM, with a resolution of 14.5 pM and a limit of detection of 47 pM.
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DOI：
10.3390/diagnostics7030045
发表时间：
2017-07-26
期刊：
Diagnostics (Basel, Switzerland)
影响因子：
--
作者：
Forsyth R;Devadoss A;Guy OJ
通讯作者：
Guy OJ
通过还原氧化石墨烯场效应晶体管生物传感器对 PNA-DNA 杂交进行超灵敏无标记检测
影响因子：
17.1
作者：
Cai, Bingjie;Wang, Shuting;Zhang, Guo-Jun
通讯作者：
Zhang, Guo-Jun
影响因子：
7.4
作者：
Gokulrangan, G;Unruh, JR;Wilson, GS
通讯作者：
Wilson, GS
影响因子：
13.3
作者：
Hao, Yufeng;Wang, Yingying;Thong, John T. L.
通讯作者：
Thong, John T. L.
影响因子：
3.4
作者：
Sinclair, D;Peters, SA
通讯作者：
Peters, SA