Molecular cloning and characterization of a lipoxygenase gene (CsLOX1) from cucumber
Molecular cloning and characterization of a lipoxygenase gene (CsLOX1) from cucumber
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黄瓜脂氧合酶基因 (CsLOX1) 的分子克隆和表征
DOI：
10.15835/nbha48411960
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发表时间：
2020-12
影响因子：
1.8
通讯作者：
Shiqiang LIU
中科院分区：
文献类型：
--
作者：
Yong ZHOU;Mingyuan XU;Wei LAI;Lianghai CHEN;Yingui YANG;Shiqiang LIU
脂氧合酶（Lipoxygenases，LOX）是一类非血红素铁酶，在植物的许多发育过程中起着重要作用。本研究从黄瓜中克隆了一个脂氧合酶基因（CsLOX 1）。CsLOX 1的编码序列（CDS）为741 bp，编码878个氨基酸残基的蛋白质，推测其定位于细胞质中。CsLOX 1含有保守的LH 2/PLAT和脂氧合酶结构域，以及代表性的38个氨基酸基序[His-（X）4-His-（X）4-His-（X）17-His-（X）8-His]。多重序列比对和系统发育分析表明，CsLOX 1与双子叶植物9-LOX的亲缘关系较近，并具有与铁原子结合的保守残基。启动子分析表明，几个发展，应力，和死亡相关的顺式作用的调控元件存在于CsLOX 1的启动子区域。通过在拟南芥中过量表达CsLOX 1来评估其功能，转基因植株雄性不育，并显示明显增加的花芽。这些结果为揭示CsLOX 1在黄瓜中的生物学作用提供了线索。
Lipoxygenases (LOXs) are non-heme iron enzymes that play crucial roles in many developmental processes during plant life, and defense responses against biotic and abiotic stresses. In this study, a lipoxygenase gene (CsLOX1) was cloned and characterized from cucumber (Cucumis sativus). The coding sequence (CDS) of CsLOX1 was 741 bp, and encoded an 878 amino-acid residue protein, which was predicted to be located in the cytoplasm. CsLOX1 contained the conserved LH2/PLAT and lipoxygenase domains, as well as the representative 38 amino acids motif [His-(X)4-His-(X)4-His-(X)17-His-(X)8-His]. Multiple sequence alignment and phylogenetic analysis indicated that CsLOX1 was closely related to other dicot 9-LOXs and posesess the essential conserved residues involved in the binding of the iron atom. Promoter analysis suggested that several development-, stress-, and hormone-related cis-acting regulatory elements were present in the promoter region of CsLOX1. The function of CsLOX1 was assessed by overexpression it in Arabidopsis, and the transgenic plants were male sterile and displayed obviously increased floral shoots. These results provide some clues for revealing the biological roles of CsLOX1 in cucumber.
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