Overexpression of an APETALA1-like gene from cucumber (Cucumis sativus L.) induces earlier flowering and abnormal leaf development in transgenic Arabidopsis
Overexpression of an APETALA1-like gene from cucumber (Cucumis sativus L.) induces earlier flowering and abnormal leaf development in transgenic Arabidopsis
复制标题
黄瓜 (Cucumis sativus L.) 中 APETALA1 样基因的过度表达可诱导转基因拟南芥提前开花和异常叶片发育
DOI：
10.1139/cjps-2018-0107
复制
发表时间：
2019-03
影响因子：
1.2
通讯作者：
Liu Shiqiang
中科院分区：
文献类型：
--
作者：
Zhou Yong;Hu Lifang;Ye Shuifeng;Jiang Lunwei;Liu Shiqiang
摘要：MADS-box蛋白是重要的转录因子，在植物发育的各个方面发挥着重要的作用，特别是在花的发育过程中。本研究对从黄瓜中分离到的CsMADS09进行了鉴定和功能鉴定。CsMADS09含有一个648bp的开放阅读框，编码215个氨基酸残基，与MADS-box蛋白AP1/FUL家族成员，特别是euAPETALA1(EuAP1)亚类有很高的序列同源性。在CsMADS09的启动子区域发现了许多与植物发育、逆境反应和激素相关的顺式元件。实时定量聚合酶链式反应结果表明，CsMADS09主要在雄花和未展开的子房等生殖组织中表达，而在根中的表达较低，仅在受精子房中可见表达。此外，CsMADS09的表达在雄花发育过程中呈下降趋势，在雌花发育过程中几乎保持不变。异源表达CsMADS09导致转基因拟南芥提前开花和叶片发育异常。本研究首次对黄瓜类AP1基因进行了功能分析，为揭示黄瓜花发育的分子机制提供了线索。
Abstract: MADS-box proteins are important transcription factors that play essential roles in various aspects of plant development, particularly in flower development. In this study, we performed the identification and functional characterization of CsMADS09 isolated from cucumber (Cucumis sativus L.). CsMADS09 contains a 648-bp open reading frame encoding 215 amino acid residues, and shares high sequence identities with the members of the AP1/FUL family of MADS-box proteins, especially the euAPETALA1 (euAP1) subclade. Many cis-elements related to plant development, stress response, and hormones were identified in the promoter region of CsMADS09. Quantitative real-time polymerase chain reaction results showed that CsMADS09 was mainly expressed in reproductive tissues such as male flowers and unexpanded ovaries, while its expression was low in roots and only traceable in fertilized ovaries. Moreover, the results revealed that CsMADS09 expression tended to decline during male flower development and stayed nearly constant during female flower development. Ectopic expression of CsMADS09 resulted in earlier flowering and abnormal leaf development in transgenic Arabidopsis. This study is the first functional analysis of an AP1-like gene from cucumber and provides some clues for revealing the molecular mechanisms of flower development in cucumber.
登录
查看更多内容
番茄（Solanum lycopersicum）MADS-box转录因子SlMBP8调节干旱、耐盐和胁迫相关基因
影响因子：
4.2
作者：
Yin Wencheng;Hu Zongli;Hu Jingtao;Zhu Zhiguo;Yu Xiaohui;Cui Baolu;Chen Guoping
通讯作者：
Chen Guoping
影响因子：
2.4
作者：
T. Chuang;Kun-Hung Li;Pei-Fang Li;Chang-Hsien Yang
通讯作者：
T. Chuang;Kun-Hung Li;Pei-Fang Li;Chang-Hsien Yang
杨树APETALA1同源物的鉴定及表达分析
影响因子：
2.6
作者：
Rao P;Gao Kai;Lei BQ;An XM
通讯作者：
An XM
影响因子：
5.6
作者：
Brendolise C;Espley RV;Lin-Wang K;Laing W;Peng Y;McGhie T;Dejnoprat S;Tomes S;Hellens RP;Allan AC
通讯作者：
Allan AC
影响因子：
16.6
作者：
Balanzà V;Martínez-Fernández I;Sato S;Yanofsky MF;Kaufmann K;Angenent GC;Bemer M;Ferrándiz C
通讯作者：
Ferrándiz C