Single-Cell Atlas of the Drosophila Leg Disc Identifies a Long Non-Coding RNA in Late Development
Single-Cell Atlas of the Drosophila Leg Disc Identifies a Long Non-Coding RNA in Late Development
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果蝇腿部发育盘单细胞图谱揭示发育后期的一种长链非编码RNA
DOI：
10.3390/ijms23126796
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发表时间：
2022-06-18
影响因子：
5.6
通讯作者：
Chan, Ting-Fung
中科院分区：
文献类型：
--
作者：
Tse, Joyce;Li, Tsz Ho;Zhang, Jizhou;Lee, Alan Chun Kit;Lee, Ivy;Qu, Zhe;Lin, Xiao;Hui, Jerome;Chan, Ting-Fung
果蝇成虫盘是研究发育基因调控的理想模型。特别是长链非编码RNA（lncRNA）由于其在基因调控中的重要作用，近年来受到广泛关注。其特定的时空表达进一步支持其在发育过程和疾病中的作用。在这项研究中，我们探讨了一种新的lncRNA在果蝇腿发育的作用，通过解剖和解离w1118三龄幼虫第三腿（L3）盘成单细胞和单核，并进行单细胞RNA测序（scRNA-seq）和单细胞分析转座酶可访问的染色质（scATAC-seq）。在三个发育时间点对L3椎间盘进行的单细胞转录组学分析揭示了不同的细胞类型，并将lncRNA：CR 33938鉴定为在发育晚期高表达的远端特异性基因。荧光原位杂交（FISH）进一步验证了这一点。scATAC-seq结果再现了单细胞转录组学景观，并阐明了不同时间点的远端细胞功能。此外，lncRNA：CR 33938在S2细胞系中的过表达增加了腿发育基因的表达，进一步阐明了其在发育中的潜在作用。
The Drosophila imaginal disc has been an excellent model for the study of developmental gene regulation. In particular, long non-coding RNAs (lncRNAs) have gained widespread attention in recent years due to their important role in gene regulation. Their specific spatiotemporal expressions further support their role in developmental processes and diseases. In this study, we explored the role of a novel lncRNA in Drosophila leg development by dissecting and dissociating w1118 third-instar larval third leg (L3) discs into single cells and single nuclei, and performing single-cell RNA-sequencing (scRNA-seq) and single-cell assays for transposase-accessible chromatin (scATAC-seq). Single-cell transcriptomics analysis of the L3 discs across three developmental timepoints revealed different cell types and identified lncRNA:CR33938 as a distal specific gene with high expression in late development. This was further validated by fluorescence in-situ hybridization (FISH). The scATAC-seq results reproduced the single-cell transcriptomics landscape and elucidated the distal cell functions at different timepoints. Furthermore, overexpression of lncRNA:CR33938 in the S2 cell line increased the expression of leg development genes, further elucidating its potential role in development.
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影响因子：
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影响因子：
2.6
作者：
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通讯作者：
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