The Spalt transcription factors regulate cell proliferation, survival and epithelial integrity downstream of the Decapentaplegic signalling pathway.
The Spalt transcription factors regulate cell proliferation, survival and epithelial integrity downstream of the Decapentaplegic signalling pathway.
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DOI：
10.1242/bio.20123038
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发表时间：
2013-01-15
期刊：
影响因子：
2.4
通讯作者：
De Celis JF
中科院分区：
文献类型：
--
作者：
Organista MF;De Celis JF
果蝇翅膀中Spalt基因的表达受Decapentaplegic信号通路的调控。这些基因通过调节静脉特异性基因的表达参与纵向翅膀静脉的形成，并参与翅膀叶片上皮中央区域细胞亲和力的建立。Spalt蛋白作为转录因子，很可能通过抑制来调节基因的表达，但它们在翅膀中的靶基因的身份仍不清楚。作为揭开由Spalt蛋白控制的遗传体系的初步步骤，我们分析了它们在翅膀发育过程中的需求，并讨论了它们在多大程度上调节这个发育系统中十足瘫痪途径的所有功能。我们确定了Spalt在细胞分裂、存活和维持上皮完整性方面的其他功能。因此，SpALT活性是促进细胞增殖所必需的，它作用于细胞周期的G2/M转换。Spalt对细胞分裂的贡献仅限于翼叶片的中央区域，因为它们不能调节翼盘外围区域的去麻木信号所引发的额外生长。此外，在暴露于高水平的Decapenta麻痹信号的细胞中，Spalt功能是维持细胞活力所必需的。Spalt功能的这一方面与Spalt表达域中JNK信号的抑制有关。最后，我们进一步描述了Spalt通过调节位于中央翼区的细胞之间的细胞亲和力来维持上皮完整性的要求。我们的结果表明，Spalt功能调节了确定的十足瘫痪信号的大部分要求，有助于建立区分翼片中央区域和外围区域的细胞性质。
The expression of the spalt genes is regulated by the Decapentaplegic signalling pathway in the Drosophila wing. These genes participate in the patterning of the longitudinal wing veins by regulating the expression of vein-specific genes, and in the establishment of cellular affinities in the central region of the wing blade epithelium. The Spalt proteins act as transcription factors, most likely regulating gene expression by repression, but the identity of their target genes in the wing is still unknown. As a preliminary step to unravel the genetic hierarchy controlled by the Spalt proteins, we have analysed their requirements during wing development, and addressed to what extent they mediate all the functions of the Decapentaplegic pathway in this developmental system. We identify additional functions for Spalt in cell division, survival, and maintenance of epithelial integrity. Thus, Spalt activity is required to promote cell proliferation, acting in the G2/M transition of the cell cycle. The contribution of Spalt to cell division is limited to the central region of the wing blade, as they do not mediate the extra growth triggered by Decapentaplegic signalling in the peripheral regions of the wing disc. In addition, Spalt function is required to maintain cell viability in cells exposed to high levels of Decapentaplegic signalling. This aspect of Spalt function is related to the repression of JNK signalling in the spalt domain of expression. Finally, we further characterise the requirements of Spalt to maintain epithelial integrity by regulating cellular affinities between cells located in the central wing region. Our results indicate that Spalt function mediates most of the requirements identified for Decapentaplegic signalling, contributing to establish the cellular qualities that differentiate central versus peripheral territories in the wing blade.
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影响因子：
4.6
作者：
Cantera, R;Lüer, K;Technau, GM
通讯作者：
Technau, GM
影响因子：
3.3
作者：
Cruz, Cristina;Glavic, Alvaro;de Celis, Jose F.
通讯作者：
de Celis, Jose F.
影响因子：
10.5
作者：
Martín-Blanco, E;Gampel, A;Martinez-Arias, A
通讯作者：
Martinez-Arias, A
DOI：
10.1073/pnas.94.11.5691
发表时间：
1997-05-27
影响因子：
11.1
作者：
Milan, M;Campuzano, S;GarciaBellido, A
通讯作者：
GarciaBellido, A
影响因子：
64.5
作者：
EDGAR, BA;OFARRELL, PH
通讯作者：
OFARRELL, PH