Preparation and characterization of two novel osteoinductive fishbone-derived biphasic calcium phosphate bone graft substitutes
Preparation and characterization of two novel osteoinductive fishbone-derived biphasic calcium phosphate bone graft substitutes
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两种新型骨诱导鱼骨源双相磷酸钙骨移植替代物的制备和表征
DOI：
10.1177/08853282221111969
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发表时间：
2022-07
期刊：
影响因子：
--
通讯作者：
Yuntong Zhang
中科院分区：
文献类型：
--
作者：
Qingfeng Zhu;Tong Chen;Jinfeng Xia;Danyu Jiang;Shaohai Wang;Yuntong Zhang
利用天然资源制备以羟基磷灰石（HA）为主要成分的异种骨移植材料的研究已有很多报道，但从动物骨中提取双相磷酸钙（HA/β-TCP）并转化为骨移植替代物的研究报道较少。本研究将两种鱼骨（萨尔莫900°C煅烧的Sa 900和Anoplopoma fimbria 800）通过一系列制备工艺制成颗粒状多孔双相磷酸钙骨移植替代物，其粒径在500 ~ 1000 μm之间。采用X射线衍射（XRD）和傅里叶变换红外光谱（FTIR）对产物的化学组成进行了表征。用扫描电镜（SEM）和压汞仪对支架的形貌和孔结构进行了比较分析。采用基于BET理论的氮气吸附法测定了材料的比表面积。同时进行细胞毒性和异位成骨实验，以研究这些材料的生物相容性和骨诱导潜力。结果表明，两种鱼骨支架材料均由不同比例的HA和β-TCP组成，材料表面存在大量大小不一的连通孔隙。An 800具有较高的总孔隙率，达到74.8%，具有较高的连通性，微孔主要分布在0.27 μm和0.12 μm处，而Sa 900具有较高的比表面积和较高的颗粒内孔隙率，纳米孔主要分布在0.07 μm处。CCK-8测定和活/死染色显示出优异的生物相容性。植入Wistar大鼠肌袋8周后，材料内孔和外周界面均观察到材料诱导的类骨质形成，表明材料具有一定的骨诱导潜力。本文还讨论了材料诱导成骨的可能机制以及材料的化学成分、表面形貌和空间结构对成骨的影响。
Many studies have reported on the conversion of natural resources into xenografts with hydroxyapatite (HA) as major component, but the extraction of biphasic calcium phosphate (HA/β-TCP) from animal bones and transformation into bone graft substitutes are rarely reported. In this research, two kinds of fish bones were made into granular porous biphasic calcium phosphate bone graft substitutes with particle sizes between 500 to 1000 μm through a series of preparation procedures ( Salmo salar calcined at 900°C named Sa900 and Anoplopoma fimbria calcined at 800°C named An800). The chemical composition was characterized by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). The morphology and porous structure of the scaffolds were comparatively analyzed by scanning electron microscopy (SEM) and mercury porosimeter. The specific surface area of materials was measured by the nitrogen adsorption technique based on BET theory. Cytotoxicity and ectopic osteogenesis were also carried out to investigate the biocompatibility and osteoinductive potential of these materials. The results showed that both fishbone-derived scaffolds were composed of HA and β-TCP with different proportions, and numerous interconnected pores with different sizes were observed at the surface of materials. An800 had higher total porosity reaching 74.8% with higher interconnectivity and micropores mostly distributed at 0.27 μm and 0.12 μm, while Sa900 had a higher specific surface area and higher intraparticle porosity with nanopores mostly distributed at 0.07 μm. CCK-8 assays and Live/dead staining demonstrated excellent biocompatibility. Material-induced osteoid formation were observed on the interface of both internal pores and periphery of materials after implantation in muscle pouch of Wistar rats for 8 weeks which indicated some extent of osteoinductive potential of materials. The possible mechanism of material-induced osteogenesis and the effects of chemical composition, surface topography, and spatial structure on osteogenesis were also discussed in this paper.
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DOI：
10.1002/jbm.b.31271
发表时间：
2009-07-01
影响因子：
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作者：
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DOI：
10.1016/j.msec.2016.06.097
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10.1016/j.msec.2012.08.014
发表时间：
2013-01-01
影响因子：
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Piccirillo, C.;Silva, M. F.;Castro, P. M. L.
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DOI：
--
发表时间：
2011
期刊：
--
影响因子：
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A. Barradas;Huipin Yuan;C. A. V. Blitterswijk;P. Habibović
DOI：
10.1016/j.dental.2015.09.008
发表时间：
2016-01
期刊：
Dental materials : official publication of the Academy of Dental Materials
影响因子：
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