Microdissection and chromosome painting of the alien chromosome in an addition line of wheat--Thinopyrum intermedium.
Microdissection and chromosome painting of the alien chromosome in an addition line of wheat--Thinopyrum intermedium.
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小麦附加系中外来染色体的显微解剖和染色体涂色 - Thinopyrum intermedium
DOI：
10.1371/journal.pone.0072564
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Hu Z
中科院分区：
文献类型：
--
作者：
Deng C;Bai L;Fu S;Yin W;Zhang Y;Chen Y;Wang RR;Zhang X;Han F;Hu Z
本研究利用染色体涂染技术对小麦-中间偃麦草异附加系TAi-27的外源染色体进行了鉴定，并对中间偃麦草三个不同染色体组的染色体进行了鉴定。中间体以TAi-27最小的外源染色体为探针，经DOP-PCR扩增后，与TAi-27和Th的中期染色体进行杂交。中间的结果表明，在TAi-27的一对最小的外源染色体的所有区域和另一对外源染色体的着丝粒周围区域均观察到杂交信号，表明来自显微切割染色体的探针具有种特异性。在Th .中间型有14条染色体的杂交信号宽而强，主要分布在着丝粒周围，9条染色体的杂交信号窄而弱，主要分布在着丝粒周围。其余染色体显示出非常弱或无信号。连续FISH/GISH检测Th .以穗状拟鹅观草（Pseudoroegneria spicata，St）染色体DNA和Js基因组特异性探针pDbH 12为探针，对中间偃麦草（Th . J基因组上无杂交信号，与Th基因组相似。比萨拉比姆。我们的研究结果表明，最小的外来染色体可能代表一个截短的染色体和重复序列的分布可能是相似的，在不同的染色体内的St基因组。然而，重复序列在Js基因组内、单个染色体内和Th不同基因组之间的分布是不同的。中间的研究结果表明，染色体涂染技术在某些植物中是可行的，可用于检测多倍体植物不同基因组中的染色体变异和重复序列分布，有助于了解多倍体植物不同基因组的进化。
In this study, chromosome painting was developed and used to identify alien chromosomes in TAi-27, a wheat - Thinopyrum intermedium addition line, and the chromosomes of the three different genomes of Th. Intermedium. The smallest alien chromosome of TAi-27 was microdissected and its DNA amplified by DOP-PCR was used as a probe to hybridize with metaphase chromosomes of TAi-27 and Th . intermedium . Results showed that hybridization signals were observed in all regions of a pair of the smallest alien chromosomes and the pericentromeric area of another pair of alien chromosomes in TAi-27, indicating that the probe from microdissected chromosome is species specific. In Th . intermedium , 14 chromosomes had wide and strong hybridization signals distributed mainly on the pericentromere area and 9 chromosomes with narrow and weak signals on the pericentromere area. The remaining chromosomes displayed a very weak or no signal. Sequential FISH/GISH on Th . intermedium chromosomes using the DNAs of microdissected chromosome, Pseudoroegneria spicata (St genome) and pDbH12 (a Js genome specific probe) as the probes indicated that the microdissected chromosome belonged to the St genome, three genomes (Js, J and St) in Th . intermedium could be distinguished, in which there is no hybridization signal on J genome that is similar to the genome of Th . bessarabicum . Our results showed that the smallest alien chromosomes may represent a truncated chromosome and the repetitive sequence distribution might be similar in different chromosomes within the St genome. However, the repetitive sequence distributions are different within the Js genome, within a single chromosome, and among different genomes in Th . intermedium . Our results suggested that chromosome painting could be feasible in some plants and useful in detecting chromosome variation and repetitive sequence distribution in different genomes of polyploidy plants, which is helpful for understanding the evolution of different genomes in polyploid plants.
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影响因子：
3.8
作者：
Nie, W.;Wang, J.;Su, W.;Wang, D.;Tanomtong, A.;Perelman, P. L.;Graphodatsky, A. S.;Yang, F.
通讯作者：
Yang, F.
影响因子：
3.1
作者：
Fedak, G;Chen, Q;Armstrong, KW
通讯作者：
Armstrong, KW
影响因子：
3.1
作者：
Fedak, G
通讯作者：
Fedak, G
染色体涂色揭示中华穿山甲（Manis pentadactyla）和马来穿山甲（Manis javanica）核型差异背后的染色体重排
影响因子：
2.6
作者：
Nie, Wenhui;Wang, Jinhuan;Yang, Fengtang
通讯作者：
Yang, Fengtang
影响因子：
3.1
作者：
LARKIN, PJ;BANKS, PM;MCINTOSH, RA
通讯作者：
MCINTOSH, RA