MicroRNAs identified in highly purified liver-derived mitochondria may play a role in apoptosis.
MicroRNAs identified in highly purified liver-derived mitochondria may play a role in apoptosis.
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DOI：
10.4161/rna.6.1.7534
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发表时间：
2009-01
期刊：
影响因子：
4.1
通讯作者：
Steer CJ
中科院分区：
文献类型：
--
作者：
Kren BT;Wong PY;Sarver A;Zhang X;Zeng Y;Steer CJ
microRNAs（miRNAs）是一类长度约为22 nt的非编码小RNA，通过与mRNA靶位点直接结合来调控基因的转录后表达。它们包括哺乳动物细胞中的1，000多种新物种，并通过几种不同的机制（包括翻译抑制和/或靶mRNA降解）调节基因表达来发挥其功能。线粒体维持并表达其自身的基因组，这与核转录和翻译装置不同。因此，它们为miRNA介导的转录后调节提供了潜在的位点。为了确定它们是否保持独特的miRNA群体，我们检查了来自成年大鼠肝脏的高度纯化和RNA酶处理的线粒体的miRNA谱。通过微阵列分析鉴定了15种miRNA，其中5种通过使用大鼠特异性探针的TaqMan® 5′核酸酶测定法确认。对miRNAs的功能分析表明，它们既不靶向线粒体基因组，也不与编码线粒体蛋白的核RNA互补。相反，与miRNA相关的miRNA似乎参与了与细胞凋亡、细胞增殖和分化相关的基因的表达。考虑到线粒体在细胞凋亡中发挥的核心作用，结果表明它们可能作为选择的miRNA的储存库，这些miRNA可能以协调的方式调节这些过程。
MicroRNAs (miRNAs) are a class of small ~22 nt noncoding (nc) RNAs that regulate gene expression post-transcriptionally by direct binding to target sites on mRNAs. They comprise more than 1,000 novel species in mammalian cells and exert their function by modulating gene expression through several different mechanisms, including translational inhibition, and/or degradation of target mRNAs. Mitochondria maintain and express their own genome, which is distinct from the nuclear transcriptional and translational apparatus. Thus, they provide a potential site for miRNA mediated post-transcriptional regulation. To determine whether they maintain a unique miRNA population, we examined the miRNA profile from highly purified and RNase treated mitochondria from adult rat liver. Fifteen miRNAs were identified by microarray analysis of which, five were confirmed by TaqMan® 5′nuclease assays using rat specific probes. Functional analysis of the miRNAs indicated that they were not targeted to the mitochondrial genome nor were they complementary to nuclear RNAs encoding mitochondrial proteins. Rather, the mitochondria-associated miRNAs appear to be involved in the expression of genes associated with apoptosis, cell proliferation, and differentiation. Given the central role that mitochondria play in apoptosis, the results suggest that they might serve as reservoirs of select miRNAs that may modulate these processes in a coordinate fashion.
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影响因子：
14.9
作者：
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影响因子：
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