Multigenerational effects of benzo[a]pyrene exposure on survival and developmental deformities in zebrafish larvae.
Multigenerational effects of benzo[a]pyrene exposure on survival and developmental deformities in zebrafish larvae.
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苯并[A] pyrene暴露对斑马鱼幼虫的生存和发育畸形的多代作用。
DOI：
10.1016/j.aquatox.2013.12.028
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发表时间：
2014-03
影响因子：
4.5
通讯作者：
Willett, Kristine L.
中科院分区：
文献类型：
--
作者：
Corrales, Jone;Thornton, Cammi;White, Mallory;Willett, Kristine L.
在水生环境中，膳食多环芳烃（PAH）暴露的不良后果知之甚少，多代发育影响暴露于多环芳烃需要探索。苯并[a]芘（BaP）是一种典型的多环芳烃，是公认的致癌物和内分泌干扰物。在这里，成年斑马鱼（F0）以1%体重的摄食率每天两次喂食0、10、114或1012 μg BaP/g饲料，持续21天。收集卵并饲养胚胎（F1），以评估死亡率和受精后（hpf）24、32、48、56、72、80和96小时孵化时间，然后在96 hpf对发育畸形进行评分。饲养F1代鱼以产生F2代，然后是F3和F4代。在F1代中，较高剂量BaP组（2.3和20 μg BaP/g鱼）的死亡率显著增加，而在F2、F3或F4代中没有差异。此外，在较高剂量的F1代存活鱼中观察到过早孵化，但在F2和F3代中未发现差异。虽然只有成年F0代苯并（a）芘处理，但这种暴露导致至少两代（F1和F2）的多代表型影响。身体形态畸形（身体、尾巴和胸鳍的形状）是观察到的最严重的异常，这些在F1代中最为极端，但仍存在于F2代中，而非F3代。颅面结构（大脑区域的长度，视和耳囊的大小，和颌骨畸形），虽然没有显着影响的F1代，出现在F2代的显着畸形。未来的工作将试图从分子上锚持久的多代表型畸形指出，在这项研究中所造成的苯并（a）芘曝光。
In the aquatic environment, adverse outcomes from dietary polycyclic aromatic hydrocarbon (PAH) exposure are poorly understood, and multigenerational developmental effects following exposure to PAHs are in need of exploration. Benzo[a]pyrene (BaP), a model PAH, is a recognized carcinogen and endocrine disruptor. Here adult zebrafish (F0) were fed 0, 10, 114, or 1012 μg BaP/g diet at a feed rate of 1% body weight twice/day for 21 days. Eggs were collected and embryos (F1) were raised to assess mortality and time to hatch at 24, 32, 48, 56, 72, 80, and 96 hours post fertilization (hpf) before scoring developmental deformities at 96 hpf. F1 generation fish were raised to produce the F2 generation followed by the F3 and F4 generations. Mortality significantly increased in the higher dose groups of BaP (2.3 and 20 μg BaP/g fish) in the F1 generation while there were no differences in the F2, F3, or F4 generations. In addition, premature hatching was observed among the surviving fish in the higher dose of the F1 generation, but no differences were found in the F2 and F3 generations. While only the adult F0 generation was BaP-treated, this exposure resulted in multigenerational phenotypic impacts on at least two generations (F1 and F2). Body morphology deformities (shape of body, tail, and pectoral fins) were the most severe abnormality observed, and these were most extreme in the F1 generation but still present in the F2 but not F3 generations. Craniofacial structures (length of brain regions, size of optic and otic vesicles, and jaw deformities), although not significantly affected in the F1 generation, emerged as significant deformities in the F2 generation. Future work will attempt to molecularly anchor the persistent multigenerational phenotypic deformities noted in this study caused by BaP exposure.
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