The role of the Kinesin-13 family protein TbKif13-2 in flagellar length control of Trypanosoma brucei.
The role of the Kinesin-13 family protein TbKif13-2 in flagellar length control of Trypanosoma brucei.
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DOI：
10.1016/j.molbiopara.2010.08.001
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发表时间：
2010-12
影响因子：
1.5
通讯作者：
Ersfeld, Klaus
中科院分区：
文献类型：
--
作者：
Chan, Kuan Yoow;Ersfeld, Klaus
布氏锥虫含有至少5个微管解聚驱动蛋白，但鞭毛驱动蛋白TbKif 13 -2只有中等的影响鞭毛动力学。解聚驱动蛋白TbKif 13 -2定位于T.布鲁塞。这种驱动蛋白的基因缺失突变体对鞭毛的长度没有可测量的影响。Myc标记的TbKif 13 -2的过表达对新鞭毛生长的初始速率和成熟鞭毛的长度具有边缘但显著的影响。TbKif 13 -2是微管解聚驱动蛋白13家族的成员，定位于布氏锥虫鞭毛的顶端。其预测的活动建议轴丝长度的调节中的作用。然而，使用基因缺失和TbKif 13 -2的过表达，我们表明，在原环T。brucei，该驱动蛋白对鞭毛长度仅具有非常有限的作用。基因缺失导致鞭毛没有显着的伸长和过度表达只有轻微的鞭毛长度和细胞分裂过程中的新鞭毛的生长速度下降。这与在利什曼原虫中的研究相反，在利什曼原虫中TbKif 13 -2同源物的过表达导致鞭毛的长度显著减少。然而，TbKif 13 -2的敲除对出现的新鞭毛的初始生长有影响。总之，我们发现TbKif 13 -2对T.布鲁塞。
Trypanosoma brucei contains at least five microtubule-depolymerising kinesins, but the flagellar kinesin TbKif13-2 has only a moderate effect on flagellar dynamics. ▶ The depolymerising kinesin TbKif13-2 is localised at the tip of the mature and new flagellum of T. brucei. ▶ A gene deletion mutant of this kinesin has no measurable effect on the length of the flagellum. ▶ Overexpression of a myc-tagged TbKif13-2 has marginal, but significant effects on the initial rate of outgrowth of the new flagellum and on the length of the mature flagellum. TbKif13-2, a member of the microtubule-depolymerising Kinesin-13 family was localised at the tip of the flagellum in Trypanosoma brucei. Its predicted activity suggested a role in the regulation of axonemal length. However, using gene deletion and overexpression of TbKif13-2 we show that, in procyclic T. brucei, this kinesin has only a very limited effect on flagellar length. Gene deletion resulted in no significant elongation of the flagellum and overexpression only slightly decreased flagellar length and the rate of growth of a new flagellum during cell division. This is in contrast to studies in Leishmania major, where overexpression of the TbKif13-2 homologue resulted in a significant length reduction of the flagellum. Knockout of TbKif13-2 has, however, an effect on the initial growth of the emerging new flagellum. In conclusion, we show that TbKif13-2 has only a marginal impact on flagellar length in T. brucei.
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