Ontogenesis of NADPH-diaphorase positive neurons in guinea pig neocortex.
Ontogenesis of NADPH-diaphorase positive neurons in guinea pig neocortex.
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豚鼠新皮质 NADPH 心肌黄酶阳性神经元的个体发生
DOI：
10.3389/fnana.2015.00011
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发表时间：
2015
影响因子：
2.9
通讯作者：
Yan XX
中科院分区：
文献类型：
--
作者：
Liu C;Yang Y;Hu X;Li JM;Zhang XM;Cai Y;Li Z;Yan XX
在哺乳动物大脑中存在两种不同类型的表达一氧化氮合酶（NOS）的中间神经元。I型神经元体积大，表现出重的烟酰胺腺嘌呤二核苷酸磷酸黄递酶（NADPH-d）组织化学反应，而II型细胞小，NADPH-d反应轻。在哺乳动物中，这些皮质神经元相对于皮质发生的起源时间在很大程度上仍不清楚。在这里，我们使用细胞出生日期和双标记方法在豚鼠中探索了这个问题。给怀孕时间的母亲进行溴脱氧尿苷（BrdU）脉冲追踪（2次剂量为50 mg/kg，间隔12 h），然后在出生后第0天（P0）、P30和P60对后代顶叶和颞叶新皮质中的NADPH-d/BrdU共定位进行定量。在E21、E28和E35的脉冲追踪刺激后，在P0、P30和P60，I型神经元被BrdU部分共标记，在三个时间组中，I型神经元占总神经元的2-11.3%。在P0时，在E21、E28、E35和E42脉冲追踪后，II型神经元被部分共标记为BrdU（对于各个时间组，占总群体的8.6%-16.5%）。在P60，发现II型神经元在E21、E28、E35、E42、E49、E56和E60/61的脉冲追逐后共表达BrdU（对于各个时间组，占总群体的4.8-11.3%）。这些结果表明，在豚鼠中，I型神经元在早期皮质生成过程中产生，而II型细胞在广泛的产前时间窗内产生，持续到出生。这些数据还表明，II型一氧化氮能神经元可能会经历一个时期的发展/分化，超过1个月，才是NADPH-d反应。
In mammalian cerebrum there exist two distinct types of interneurons expressing nitric oxide synthase (NOS). Type I neurons are large in size and exhibit heavy nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) histochemical reaction, while type II cells are small with light NADPH-d reactivity. The time of origin of these cortical neurons relative to corticogenesis remains largely unclear among mammals. Here we explored this issue in guinea pigs using cell birth-dating and double-labeling methods. Bromodeoxyuridine (BrdU) pulse-chasing (2 doses at 50 mg/kg, 12 h apart) was given to time-pregnant mothers, followed by quantification of NADPH-d/BrdU colocalization in the parietal and temporal neocortex in offspring at postnatal day 0 (P0), P30 and P60. Type I neurons were partially colabeled with BrdU at P0, P30 and P60 following pulse-chasing at embryonic day 21 (E21), E28 and E35, varied from 2–11.3% of total population of these neurons for the three time groups. Type II neurons were partially colabeled for BrdU following pulse-chasing at E21, E28, E35 and E42 at P0 (8.6%–16.5% of total population for individual time groups). At P60, type II neurons were found to co-express BrdU (4.8–11.3% of total population for individual time groups) following pulse-chasing at E21, E28, E35, E42, E49, E56 and E60/61. These results indicate that in guinea pigs type I neurons are generated during early corticogenesis, whereas type II cells are produced over a wide prenatal time window persisting until birth. The data also suggest that type II nitrinergic neurons may undergo a period of development/differentiation, for over 1 month, before being NADPH-d reactive.
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影响因子：
2.9
作者：
AOKI, C;FENSTEMAKER, S;GO, CG
通讯作者：
GO, CG
影响因子：
5.3
作者：
Cai, Yan;Xiong, Kun;Chu, Yaping;Luo, Duan-Wu;Luo, Xue-Gang;Yuan, Xian-Yui;Struble, Robert G.;Clough, Richard W.;Spencer, Dennis D.;Williamson, Anne;Kordower, Jeffrey H.;Patrylo, Peter R.;Yan, Xiao-Xin
通讯作者：
Yan, Xiao-Xin
影响因子：
64.8
作者：
ALTMAN, J;DAS, GD
通讯作者：
DAS, GD
影响因子：
2.9
作者：
Cruz-Rizzolo, RJ;Horta-Júnior, JAC;Casatti, CA
通讯作者：
Casatti, CA
影响因子：
3.8
作者：
Ceccatelli, S
通讯作者：
Ceccatelli, S