Protein-driven RNA nanostructured devices that function in vitro and control mammalian cell fate.
Protein-driven RNA nanostructured devices that function in vitro and control mammalian cell fate.
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DOI：
10.1038/s41467-017-00459-x
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发表时间：
2017-09-14
影响因子：
16.6
通讯作者：
Saito H
中科院分区：
文献类型：
--
作者：
Shibata T;Fujita Y;Ohno H;Suzuki Y;Hayashi K;Komatsu KR;Kawasaki S;Hidaka K;Yonehara S;Sugiyama H;Endo M;Saito H
核酸纳米技术在未来的治疗应用中具有巨大的潜力。然而，通过检测和放大蛋白质信号来控制细胞命运的纳米结构器件的构建仍然是一个挑战。在这里，我们设计并构建了蛋白质驱动的rna纳米结构装置，该装置通过rna结合蛋白诱导的体外构象变化来驱动，并通过rna -蛋白相互作用介导的蛋白质组装来调节哺乳动物细胞的命运。RNA纳米结构的构象和功能受RNA结合蛋白信号的动态控制。蛋白质响应RNA纳米器件是在细胞内使用RNA仅递送构建的，这可能为构建功能性RNA-蛋白质纳米结构提供了一种安全的工具。此外，设计的RNA支架在纳米尺度上控制凋亡调节蛋白的组装和寡聚化，通过特异性RNA -蛋白相互作用选择性地杀死靶细胞。这些发现表明，合成RNA纳米器件可以作为分子机器人来检测信号和定位靶蛋白，诱导RNA构象变化，并编程哺乳动物的细胞行为。核酸纳米技术在未来的治疗应用中具有巨大的潜力。在这里，作者构建了蛋白质驱动的RNA纳米结构，可以在哺乳动物细胞内发挥作用并调节细胞命运。
Nucleic acid nanotechnology has great potential for future therapeutic applications. However, the construction of nanostructured devices that control cell fate by detecting and amplifying protein signals has remained a challenge. Here we design and build protein-driven RNA-nanostructured devices that actuate in vitro by RNA-binding-protein-inducible conformational change and regulate mammalian cell fate by RNA–protein interaction-mediated protein assembly. The conformation and function of the RNA nanostructures are dynamically controlled by RNA-binding protein signals. The protein-responsive RNA nanodevices are constructed inside cells using RNA-only delivery, which may provide a safe tool for building functional RNA–protein nanostructures. Moreover, the designed RNA scaffolds that control the assembly and oligomerization of apoptosis-regulatory proteins on a nanometre scale selectively kill target cells via specific RNA–protein interactions. These findings suggest that synthetic RNA nanodevices could function as molecular robots that detect signals and localize target proteins, induce RNA conformational changes, and programme mammalian cellular behaviour. Nucleic acid nanotechnology has great potential for future therapeutic applications. Here the authors build protein-driven RNA nanostructures that can function within mammalian cells and regulate the cell fate.
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影响因子：
3.7
作者：
Banning C;Votteler J;Hoffmann D;Koppensteiner H;Warmer M;Reimer R;Kirchhoff F;Schubert U;Hauber J;Schindler M
通讯作者：
Schindler M
影响因子：
56.9
作者：
Chworos, A;Severcan, I;Jaeger, L
通讯作者：
Jaeger, L
影响因子：
16.6
作者：
通讯作者：
--
影响因子：
56.9
作者：
Geary, Cody;Rothemund, Paul W. K.;Andersen, Ebbe S.
通讯作者：
Andersen, Ebbe S.
影响因子：
15
作者：
Li, Juan;Zheng, Cheng;Cansiz, Sena;Wu, Cuichen;Xu, Jiehua;Cui, Cheng;Liu, Yuan;Hou, Weijia;Wang, Yanyue;Zhang, Liqin;Teng, I-ting;Yang, Huang-Hao;Tan, Weihong
通讯作者：
Tan, Weihong