Alterations in intestinal microbiota diversity, composition, and function in patients with sarcopenia.
Alterations in intestinal microbiota diversity, composition, and function in patients with sarcopenia.
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石棺减少症患者肠道微生物区系多样性、组成和功能的改变。
DOI：
10.1038/s41598-021-84031-0
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发表时间：
2021-02-25
影响因子：
4.6
通讯作者：
Qu X
中科院分区：
文献类型：
--
作者：
Kang L;Li P;Wang D;Wang T;Hao D;Qu X
人类粪便样本的16 S rRNA测序在识别与衰老和疾病相关的微生物组变化方面取得了巨大成功。许多研究已经描述了与身体虚弱和老年人养老院居住相对应的微生物变化。已经假设了肠道-肌肉轴，微生物组通过该肠道-肌肉轴影响骨骼肌生长/功能。然而，微生物组尚未在肌肉减少症中进行检查。在这里，我们收集了60名健康对照（CON）和27名肌肉减少症（Case）/可能肌肉减少症（preCase）个体的粪便样本，并使用16 S rRNA测序分析了肠道微生物群。我们观察到病例和preCase样本中微生物多样性的总体减少。属毛螺菌属，Fusicantenibacter，Roseburia，真杆菌属，和毛梭菌属-已知的丁酸生产者-显着不太丰富的情况下和preCase科目，而乳酸杆菌更丰富。在病例受试者中代表性不足的功能途径包括许多转运蛋白和苯丙氨酸、酪氨酸和色氨酸生物合成，这表明蛋白质加工和营养物质转运可能受损。相反，脂多糖生物合成在病例和前病例受试者中过度表达，表明肌肉减少症与促炎性宏基因组相关。这些分析表明，肠道微生物群的结构和功能改变可能导致骨骼肌减少症中骨骼肌质量和功能的丧失。
16S rRNA sequencing of human fecal samples has been tremendously successful in identifying microbiome changes associated with both aging and disease. A number of studies have described microbial alterations corresponding to physical frailty and nursing home residence among aging individuals. A gut-muscle axis through which the microbiome influences skeletal muscle growth/function has been hypothesized. However, the microbiome has yet to be examined in sarcopenia. Here, we collected fecal samples of 60 healthy controls (CON) and 27 sarcopenic (Case)/possibly sarcopenic (preCase) individuals and analyzed the intestinal microbiota using 16S rRNA sequencing. We observed an overall reduction in microbial diversity in Case and preCase samples. The genera Lachnospira, Fusicantenibacter, Roseburia, Eubacterium, and Lachnoclostridium—known butyrate producers—were significantly less abundant in Case and preCase subjects while Lactobacillus was more abundant. Functional pathways underrepresented in Case subjects included numerous transporters and phenylalanine, tyrosine, and tryptophan biosynthesis suggesting that protein processing and nutrient transport may be impaired. In contrast, lipopolysaccharide biosynthesis was overrepresented in Case and PreCase subjects suggesting that sarcopenia is associated with a pro-inflammatory metagenome. These analyses demonstrate structural and functional alterations in the intestinal microbiota that may contribute to loss of skeletal muscle mass and function in sarcopenia.
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影响因子：
3.7
作者：
Biagi E;Nylund L;Candela M;Ostan R;Bucci L;Pini E;Nikkïla J;Monti D;Satokari R;Franceschi C;Brigidi P;De Vos W
通讯作者：
De Vos W
影响因子：
--
作者：
Biagi, Elena;Candela, Marco;Fairweather-Tait, Susan;Franceschi, Claudio;Brigidi, Patrizia
通讯作者：
Brigidi, Patrizia
影响因子：
2.6
作者：
He, F;Ouwehand, AC;Salminen, S
通讯作者：
Salminen, S
影响因子：
4.6
作者：
Collins KH;Paul HA;Hart DA;Reimer RA;Smith IC;Rios JL;Seerattan RA;Herzog W
通讯作者：
Herzog W
影响因子：
64.8
作者：
Claesson, Marcus J.;Jeffery, Ian B.;O'Toole, Paul W.
通讯作者：
O'Toole, Paul W.