A Structurally and Functionally Biomimetic Biphasic Scaffold for Intervertebral Disc Tissue Engineering.
A Structurally and Functionally Biomimetic Biphasic Scaffold for Intervertebral Disc Tissue Engineering.
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DOI：
10.1371/journal.pone.0131827
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Chan BP
中科院分区：
文献类型：
--
作者：
Choy AT;Chan BP
组织工程为椎间盘（IVD）退变的治疗带来了很大希望。虽然椎间盘髓核和纤维环的支架已被广泛研究，但使用天然细胞外基质的真正仿生且具有力学功能的双相支架仍有待开发。在此，用胶原蛋白和糖胺聚糖（GAGs）——椎间盘内两种最丰富的细胞外基质成分——制造了一种双相支架。制造完成后，对该支架进行了表征，并与天然椎间盘进行了对比。双相支架由构成类似髓核核心的胶原蛋白 - GAG共沉淀物组成，并被包裹在多个光化学交联的胶原蛋白膜薄片中，这些薄片构成类似纤维环的薄片。在力学测试中，与天然椎间盘所呈现的99%的恢复率相比，我们制造的椎间盘高度以一种不依赖纤维环的方式恢复了约82 - 89%。椎间盘高度恢复的不依赖纤维环的特性表明，制造的髓核核心的流体置换功能可能模拟了天然椎间盘这一迄今为止独特的特征。由10个类似纤维环的薄片组成的双相支架在各种设计中具有最佳的整体力学性能，因为它们在大多数方面与天然椎间盘相似，包括蠕变和恢复过程中的弹性顺应性以及恢复过程中的粘性顺应性。然而，所有双相支架的动态力学性能（包括动态刚度和阻尼因子）与天然椎间盘相似。这项研究有助于为椎间盘组织工程合理设计和开发一种仿生且力学上可行的双相支架。
Tissue engineering offers high hopes for the treatment of intervertebral disc (IVD) degeneration. Whereas scaffolds of the disc nucleus and annulus have been extensively studied, a truly biomimetic and mechanically functional biphasic scaffold using naturally occurring extracellular matrix is yet to be developed. Here, a biphasic scaffold was fabricated with collagen and glycosaminoglycans (GAGs), two of the most abundant extracellular matrix components in the IVD. Following fabrication, the scaffold was characterized and benchmarked against native disc. The biphasic scaffold was composed of a collagen-GAG co-precipitate making up the nucleus pulposus-like core, and this was encapsulated in multiple lamellae of photochemically crosslinked collagen membranes comprising the annulus fibrosus-like lamellae. On mechanical testing, the height of our engineered disc recovered by ~82-89% in an annulus-independent manner, when compared with the 99% recovery exhibited by native disc. The annulus-independent nature of disc height recovery suggests that the fluid replacement function of the engineered nucleus pulposus core might mimic this hitherto unique feature of native disc. Biphasic scaffolds comprised of 10 annulus fibrosus-like lamellae had the best overall mechanical performance among the various designs owing to their similarity to native disc in most aspects, including elastic compliance during creep and recovery, and viscous compliance during recovery. However, the dynamic mechanical performance (including dynamic stiffness and damping factor) of all the biphasic scaffolds was similar to that of the native discs. This study contributes to the rationalized design and development of a biomimetic and mechanically viable biphasic scaffold for IVD tissue engineering.
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影响因子：
4.1
作者：
Li, Chun-hei;Chik, Tsz-Kit;Chan, Barbara P.
通讯作者：
Chan, Barbara P.
影响因子：
4.1
作者：
Park, Sang-Hyug;Gil, Eun Seok;Kaplan, David L.
通讯作者：
Kaplan, David L.
影响因子：
--
作者：
Nesti LJ;Li WJ;Shanti RM;Jiang YJ;Jackson W;Freedman BA;Kuklo TR;Giuliani JR;Tuan RS
通讯作者：
Tuan RS
影响因子：
14
作者：
Cheng, Hiu-Wa;Luk, Keith D. K.;Chan, Barbara P.
通讯作者：
Chan, Barbara P.
DOI：
10.1073/pnas.1107094108
发表时间：
2011-08-09
影响因子：
11.1
作者：
Bowles, Robby D.;Gebhard, Harry H.;Bonassar, Lawrence J.
通讯作者：
Bonassar, Lawrence J.