Norovirus-specific immunoglobulin A in breast milk for protection against norovirus-associated diarrhea among infants.
Norovirus-specific immunoglobulin A in breast milk for protection against norovirus-associated diarrhea among infants.
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母乳中的诺如病毒特异性免疫球蛋白 A 可预防婴儿诺如病毒相关腹泻。
DOI：
10.1016/j.eclinm.2020.100561
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发表时间：
2020-10
影响因子：
15.1
通讯作者：
Saito M
中科院分区：
文献类型：
--
作者：
Labayo HKM;Pajuelo MJ;Tohma K;Ford-Siltz LA;Gilman RH;Cabrera L;Mayta H;Sanchez GJ;Cornejo AT;Bern C;Dapat C;Nochi T;Parra GI;Oshitani H;Saito M
诺如病毒（NV）可引起婴儿急性胃肠炎。体液和粪便免疫球蛋白A（伊加）反应与NV的保护相关;然而，母乳伊加对NV感染和相关腹泻的作用仍然未知。本研究旨在评估母乳中NV特异性伊加（NV-IgA）的保护作用。从参加2016-2017年秘鲁出生队列研究的母亲中收集的95份母乳样本通过ELISA使用GII·4变体和非GII·4基因型病毒样颗粒（VLP）检测总伊加和NV-IgA。根据婴儿的NV感染和腹泻状态将母乳样品分组：NV阳性伴腹泻（NV+D+，n=18）; NV阳性不伴腹泻（NV+D-，n=37）;和NV阴性不伴腹泻（NV-D-，n=40）。比较各组NV-IgA阳性率和滴度。根据NV-IgA对GII·4变异体和非GII·4基因型VLP的比率的相关性来估计交叉反应性。NV-IgA对各种VLP的阳性率较高，尤其是对GII的阳性率（89-100%）。NV+D-组对GI·1 VLP的NV-IgA阳性百分比（89% vs. 61%，p=0·03）和中位滴度（1：100 vs. 1：50，p=0·03）高于NV+D+组。不同GII·4变异体之间的相关性较高（0.87），基因组之间的相关性较低（0.23 - 0.37）。母乳中NV-IgA阳性率和滴度高的母亲有NV感染的婴儿，其腹泻症状减轻。提示诺如病毒的遗传多样性与其抗原性有关。资助国立卫生研究院过敏症和传染病研究所：1 R 01 AI 108695 - 01 A1和日本科学促进协会（促进国际联合研究B）：19 KK 0241
Norovirus (NV) causes acute gastroenteritis in infants. Humoral and fecal immunoglobulin A (IgA) responses have been correlated with protection against NV; however, the role of breast milk IgA against NV infection and associated diarrhea is still unknown. This study aimed to evaluate the protective role of NV-specific IgA (NV-IgA) in breast milk. Ninety-five breast milk samples collected from mothers enrolled in a 2016–2017 Peruvian birth cohort study were tested for total IgA and NV-IgA by ELISA using GII·4 variants and non-GII·4 genotype virus-like particles (VLPs). Breast milk samples were grouped according to the NV infection and diarrheal status of infants: NV positive with diarrhea (NV+D+, n=18); NV positive without diarrhea (NV+D-, n=37); and NV negative without diarrhea (NV-D-, n=40). The percent positivity and titer of NV-IgA were compared among groups. The cross-reactivity was estimated based on the correlation of ratio between NV-IgA against GII·4 variants and non-GII·4 genotype VLPs. NV-IgA had high positivity rates against different VLPs, especially against GII (89–100%). The NV+D- group had higher percent positivity (89% vs. 61%, p=0·03) and median titer (1:100 vs 1:50, p=0·03) of NV-IgA than the NV+D+ group against GI·1 VLPs. A relatively high correlation between different GII·4 variants (0·87) and low correlation between genogroups (0·23–0·37) were observed. Mothers with high positivity rates and titers of NV-IgA in breast milk had NV infected infants with reduced diarrheal symptoms. Antigenic relatedness to the genetic diversity of human norovirus was suggested. Funding National Institute of Allergy and Infectious Diseases, National Institute of Health: 1R01AI108695–01A1 and the Japan Society for the Promotion of Science (Fostering Joint International Research B):19KK0241
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影响因子：
3.6
作者：
Hemming M;Räsänen S;Huhti L;Paloniemi M;Salminen M;Vesikari T
通讯作者：
Vesikari T
影响因子：
9.4
作者：
MCLEAN, BS;HOLMES, IH
通讯作者：
HOLMES, IH
影响因子：
6.4
作者：
MIOTTI, PG;GILMAN, RH;YOLKEN, RH
通讯作者：
YOLKEN, RH
影响因子：
6.4
作者：
Jiang, X;Huang, PW;Pickering, LK
通讯作者：
Pickering, LK
影响因子：
6.7
作者：
Parra GI;Squires RB;Karangwa CK;Johnson JA;Lepore CJ;Sosnovtsev SV;Green KY
通讯作者：
Green KY