Rice dwarf and low tillering 10 (OsDLT10) regulates tiller number by monitoring auxin homeostasis.
Rice dwarf and low tillering 10 (OsDLT10) regulates tiller number by monitoring auxin homeostasis.
复制标题
水稻矮化和低分蘖 10 (OsDLT10) 通过监测生长素稳态来调节分蘖数量。
DOI：
10.1016/j.plantsci.2020.110502
复制
发表时间：
2020-08
期刊：
影响因子：
5.2
通讯作者：
Xiaodeng Zhan
中科院分区：
文献类型：
--
作者：
Xiaoxia Wen;Lianping Sun;Yuyu Chen;Pao Xue;Qinqin Yang;Beifang Wang;Ning Yu;Yongrun Cao;Yue Zhang;Ke Gong;Weixun Wu;Daibo Chen;Liyong Cao;Shihua Cheng;Yingxin Zhang;Xiaodeng Zhan
分蘖数是水稻重要的农艺性状，直接影响水稻有效穗数和产量形成。本研究报道了一个半矮化低分蘖突变体Osdlt10（矮化低分蘖10），该突变体表现出分蘖数减少、半矮化、粒宽增大、结实率低、叶尖卷曲等一系列农艺性状异常。表型观察表明，Osdlt10突变体在分蘖芽形成方面存在缺陷，在分蘖期生长缓慢。基于图谱的克隆揭示了 LOC_Os10g41310 是 OsDLT10 的负责基因，随后使用 CRISPR/Cas9 系统和补充实验证明了这一点。表达谱分析表明OsDLT10主要表达于茎节、腋芽和叶鞘的基本部分、叶枕。激素处理研究表明，极高浓度的外源生长素可以抑制OsDLT10的表达。 Osdlt10突变体茎节基部和腋芽内源生长素含量显着降低。结果表明OsDLT10与生长素相关。 qPCR分析结果进一步表明，生长素转运基因（PINs）和早期反应基因（IAA）的表达水平显着增加。 Osdlt10突变体中WUS-like和FON1的表达水平显着降低。这些结果表明，OsDLT10 在影响分蘖数方面发挥着关键作用，可能与激素信号和 WUS-CLV 途径相关，从而调节水稻腋芽的发育。
Tiller number is a crucial agronomic trait that directly affects the number of effective panicles and yield formation in rice. Here, we report a semi-dwarf and low tillering mutantOsdlt10(dwarf and low tillering 10) that exhibited reduced tiller number, semi-dwarfism, increased grain width, low seed-setting rate, curled leaf tip and a series of abnormalities of agronomic traits. Phenotypic observations showed thatOsdlt10mutants had defects in tiller bud formation and grew slowly at the tillering stage. Map-based cloning revealed thatLOC_Os10g41310was the responsible gene forOsDLT10, which was subsequently demonstrated using the CRISPR/Cas9 system and a complementary experiment. Expression pattern analysis indicated thatOsDLT10was primarily expressed in the stem node, the basic part of axillary bud and leaf sheath, pulvinus. The hormone treatment investigation indicated that extremely high of exogenous auxin concentrations can inhibit the expression ofOsDLT10. Endogenous auxin content decreased significantly at the base of stem node and axillary bud inOsdlt10mutants. The results showed thatOsDLT10was related to auxin. qPCR analysis results further showed that the expression levels of auxin transport genes (PINs) and early response genes (IAAs) were significantly increased. The expression levels of WUS-like andFON1were substantially decreased in theOsdlt10mutants. These results revealed thatOsDLT10played a critical role in influencing tiller number, likely in association with hormone signals and the WUS-CLV pathway, to regulate axillary bud development in rice.
登录
查看更多内容
DOI：
10.1111/j.1365-313x.2006.02916.x
发表时间：
2006-12
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Junhuang Zou;Shuying Zhang;Weiping Zhang;Gang Li;Zongxiang Chen;W. Zhai;Xianfeng Zhao;Xuebiao Pan-Xuebia
通讯作者：
Junhuang Zou;Shuying Zhang;Weiping Zhang;Gang Li;Zongxiang Chen;W. Zhai;Xianfeng Zhao;Xuebiao Pan-Xuebia
OsMADS57 和 OsTB1 之间的相互作用通过 DWARF14 调节水稻分蘖
影响因子：
16.6
作者：
通讯作者：
--
REL2，一种编码未知功能蛋白的基因，包含控制水稻卷叶的 DUF630 和 DUF632 结构域
DOI：
10.1186/s12284-016-0105-6
发表时间：
2016-12
期刊：
Rice (New York, N.Y.)
影响因子：
--
作者：
Yang SQ;Li WQ;Miao H;Gan PF;Qiao L;Chang YL;Shi CH;Chen KM
通讯作者：
Chen KM
影响因子：
3.1
作者：
Yi Zhang;J. Mao;Kun Yang;Yunfeng Li;Jian Zhang;Yuanxiang Huang;Fucheng Shen;Chaochao Zhang
通讯作者：
Yi Zhang;J. Mao;Kun Yang;Yunfeng Li;Jian Zhang;Yuanxiang Huang;Fucheng Shen;Chaochao Zhang
影响因子：
64.5
作者：
Nemhauser, Jennifer L.;Hong, Fangxin;Chory, Joanne
通讯作者：
Chory, Joanne