Concentrated growth factor combined with iRoot BP Plus promotes inflamed pulp repair: an in vitro and in vivo study.
Concentrated growth factor combined with iRoot BP Plus promotes inflamed pulp repair: an in vitro and in vivo study.
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DOI：
10.1186/s12903-023-02903-5
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发表时间：
2023-04-19
期刊：
影响因子：
2.9
通讯作者：
Liu, Hongyan
中科院分区：
文献类型：
--
作者：
Zeng, Qian;Zhou, Can;Li, Mengjie;Qiu, Yu;Wei, Xi;Liu, Hongyan
血小板浓缩物与硅酸钙骨水泥结合可促进修复性牙本质的形成。然而，很少有研究报道它们对牙髓炎症的影响。本研究旨在评价浓缩生长因子(CGF)联合iRoot BP Plus对体外培养的炎性人牙髓干细胞(HDPSCs)和体内炎症大鼠牙髓的影响。用细胞计数试剂盒检测50%CGF和25%iRoot BP Plus处理后第1、4、7天HDPSCs的增殖情况；实时定量聚合酶链式反应分析第1天炎症和第14天分化相关基因的表达。将暴露的大鼠上磨牙牙髓内注射10 mg/mL脂多糖，并直接用含或不含iRoot BP Plus提取物的CGF膜封盖，分别持续1、7、28d。对牙齿进行组织学分析和免疫组织化学染色。联合治疗后第4天和第7天，炎性hDPSC的增殖率明显高于其他治疗组(P <； 0.05)。IL-1β、IL-6和肿瘤坏死因子-α水平在炎症性hDPSCs中升高，但经CGF和iRoot BP Plus提取物处理后下降，而IL-4和IL-10的表达模式相反。CGF和iRoot BP Plus提取物联合处理后，牙齿发育相关基因OCN、Runx2和ALP的表达显著增强。在大鼠牙髓中，CGF和CGF-iRoot BP Plus组的平均炎症评分较内毒素组显著降低(P <； 0.05)，且CGF-iRoot BP Plus组修复性牙本质多于CGF组和BP组。免疫组织化学染色显示CGF-iRoot BP Plus组第1天的M1巨噬细胞较少，第7天的M2巨噬细胞较多。与单独使用CGF或iRoot BP Plus相比，CGF和iRoot BP Plus在抗炎潜力和促进牙髓愈合方面显示出协同作用。
Platelet concentrates combined with calcium silicate cements may promote reparative dentin formation. However, few studies have reported their effect on dental pulp inflammation. This study aimed to evaluate the effects of concentrated growth factor (CGF) combined with iRoot BP Plus on inflammatory human dental pulp stem cells (hDPSCs) in vitro and inflamed pulp in rats in vivo. The proliferation of LPS-stimulated hDPSCs treated with 50% CGF with/without 25% iRoot BP Plus was evaluated using Cell Counting Kit-8 on days 1, 4 and 7. The expression of genes associated with inflammation on day 1 and differentiation on day 14 was analysed by real-time polymerase chain reaction. The exposed pulp of rat maxillary molars was injected with 10 mg/mL LPS and directly capped with CGF membrane with/without iRoot BP Plus extract for 1, 7 and 28 days. The teeth were subjected to histologic analyses and immunohistochemistry. The proliferation rates of the inflammatory hDPSCs after the combination treatment were significantly higher than those after the other treatments on days 4 and 7 (P < 0.05). IL-1β, IL-6, and TNF-α levels were increased in inflammatory hDPSCs but decreased after treatment with CGF combined with iRoot BP Plus extract, whereas IL-4 and IL-10 showed the opposite expression patterns. Expression of the odontogenesis-related genes OCN, Runx2, and ALP was dramatically enhanced by combined treatment with CGF and iRoot BP Plus extract. In rat pulp, the average inflammation scores of the CGF and CGF-iRoot BP Plus groups significantly decreased in comparison with those of the LPS group (P < 0.05), and the CGF-iRoot BP Plus group had more reparative dentin than the CGF and BP groups. Immunohistochemical staining showed fewer M1 macrophages on day 1 and more M2 macrophages on day 7 in the CGF-iRoot BP Plus group than in the other groups. The combination of CGF and iRoot BP Plus showed a synergistic effect on anti-inflammatory potential and promoted greater pulp healing than CGF or iRoot BP Plus alone.
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