Genome-Wide Identification and Expression Analysis of the 14-3-3 Gene Family in Mango (Mangifera indica L.).
Genome-Wide Identification and Expression Analysis of the 14-3-3 Gene Family in Mango (Mangifera indica L.).
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芒果 14-3-3 基因家族的全基因组鉴定和表达分析
DOI：
10.3390/ijms23031593
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发表时间：
2022-01-29
影响因子：
5.6
通讯作者：
Luo C
中科院分区：
文献类型：
--
作者：
Xia L;He X;Huang X;Yu H;Lu T;Xie X;Zeng X;Zhu J;Luo C
Mi14-3-3基因家族成员与广泛参与植物激素信号转导和生理相关代谢的靶蛋白相互作用，在植物生长、发育和逆境响应中发挥重要作用。在这项研究中，通过对芒果 (Mangifera indica L.) 基因组的生物信息学分析，鉴定了 14-3-3s 家族成员。系统分析了这些基因的基因结构、染色体分布、遗传进化和表达模式及其蛋白质的理化性质和保守基序。结果鉴定出芒果基因组中 14-3-3 基因家族的 16 个成员。这些成员在染色体上的分布并不均匀，基因结构分析表明，不同成员之间的基因序列长度和内含子数量差异很大。蛋白序列分析表明Mi14-3-3蛋白具有相似的理化性质和二级、三级结构，蛋白亚细胞定位表明Mi14-3-3家族蛋白均定位于细胞核。 Mi14-3-3的序列分析表明，所有Mi14-3-3蛋白都含有典型的保守PFAM00244结构域，启动子序列分析表明，Mi14-3-3启动子含有多个激素、应激和光响应的顺式调控元件。表达分析表明，14-3-3基因在芒果所有组织中均有表达，但表达模式不同。干旱、盐和低温胁迫影响14-3-3基因的表达水平，不同的14-3-3基因对这些胁迫有不同的响应。本研究为进一步研究Mi14-3-3家族成员的功能和调控提供参考。
Members of the Mi14-3-3 gene family interact with target proteins that are widely involved in plant hormone signal transduction and physiology-related metabolism and play important roles in plant growth, development and stress responses. In this study, 14-3-3s family members are identified by the bioinformatic analysis of the mango (Mangifera indica L.) genome. The gene structures, chromosomal distributions, genetic evolution, and expression patterns of these genes and the physical and chemical properties and conserved motifs of their proteins are analysed systematically. The results identified 16 members of the 14-3-3 genes family in the mango genome. The members were not evenly distributed across the chromosomes, and the gene structure analysis showed that the gene sequence length and intron number varied greatly among the different members. Protein sequence analysis showed that the Mi14-3-3 proteins had similar physical and chemical properties and secondary and tertiary structures, and protein subcellular localization showed that the Mi14-3-3 family proteins were localized to the nucleus. The sequence analysis of the Mi14-3-3s showed that all Mi14-3-3 proteins contain a typical conserved PFAM00244 domain, and promoter sequence analysis showed that the Mi14-3-3 promoters contain multiple hormone-, stress-, and light-responsive cis-regulatory elements. Expression analysis showed that the 14-3-3 genes were expressed in all tissues of mango, but that their expression patterns were different. Drought, salt and low temperature stresses affected the expression levels of 14-3-3 genes, and different 14-3-3 genes had different responses to these stresses. This study provides a reference for further studies on the function and regulation of Mi14-3-3 family members.
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影响因子：
4.3
作者：
Li, Xuyan;Dhaubhadel, Sangeeta
通讯作者：
Dhaubhadel, Sangeeta
影响因子：
14.9
作者：
Bailey TL;Johnson J;Grant CE;Noble WS
通讯作者：
Noble WS
影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.
影响因子：
4.6
作者：
Deshpande AB;Anamika K;Jha V;Chidley HG;Oak PS;Kadoo NY;Pujari KH;Giri AP;Gupta VS
通讯作者：
Gupta VS
影响因子：
7.7
作者：
Keicher, Jutta;Jaspert, Nina;Oecking, Claudia
通讯作者：
Oecking, Claudia