Apibacter adventoris gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from honey bees.

Apibacter adventoris gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from honey bees.
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DOI:
10.1099/ijsem.0.000882
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发表时间:
2016-03
影响因子:
2.8
通讯作者:
Moran NA
Moran NA
中科院分区:
生物学3区
文献类型:
--
作者:
Kwong WK;Moran NA

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蜜蜂和大黄蜂拥有一小部分确定的肠道细菌。从东亚的两种蜜蜂中分离出与蜜蜂肠道微生物菌群的16 S rRNA基因调查序列相匹配的菌株。这些菌株是嗜中性的,非色素,过氧化氢酶阳性和氧化酶阴性。主要脂肪酸为异C15:0、异C17:0 3-OH、C16:0和C16:0 3-OH。DNA G+C含量为29- 31mol%。 它们的16 S rRNA基因序列与所描述的最近的亲属有87%的同一性。使用20个蛋白质编码基因的系统发育重建表明,这些蜜蜂来源的菌株形成了一个高度支持的单系分支,姐妹分支包含属金黄杆菌属和嗜热金属的物种在家庭黄杆菌科的拟杆菌门。根据表型和基因型特征,我们建议将这些菌株归入一个新的属和种:Apibacter advenergen. nov.,模式菌株为wkB 301 T(= NRRL B-65307 T = NCIMB 14986 T)。
Honey bees and bumble bees harbour a small, defined set of gut bacterial associates. Strains matching sequences from 16S rRNA gene surveys of bee gut microbiotas were isolated from two honey bee species from East Asia. These isolates were mesophlic, non-pigmented, catalase-positive and oxidase-negative. The major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, C16 : 0 and C16 : 0 3-OH. The DNA G+C content was 29–31 mol%. They had ∼87 % 16S rRNA gene sequence identity to the closest relatives described. Phylogenetic reconstruction using 20 protein-coding genes showed that these bee-derived strains formed a highly supported monophyletic clade, sister to the clade containing species of the genera Chryseobacterium and Elizabethkingia within the family Flavobacteriaceae of the phylum Bacteroidetes. On the basis of phenotypic and genotypic characteristics, we propose placing these strains in a novel genus and species: Apibacter adventoris gen. nov., sp. nov. The type strain of Apibacter adventoris is wkB301T ( = NRRL B-65307T = NCIMB 14986T).
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