Deep sequencing and ecological characterization of gut microbial communities of diverse bumble bee species.
Deep sequencing and ecological characterization of gut microbial communities of diverse bumble bee species.
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DOI：
10.1371/journal.pone.0118566
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Cameron SA
中科院分区：
文献类型：
--
作者：
Lim HC;Chu CC;Seufferheld MJ;Cameron SA
大黄蜂的肠道细菌群落与对病原体的防御相关。进一步了解这种宿主-微生物关联至关重要，因为熊蜂目前正在经历全球种群数量下降，部分原因可能是突发疾病。在这项研究中，我们使用焦磷酸测序和群落指纹分析 (ARISA) 来表征熊熊系统发育中九个熊物种的肠道微生物群落。总体而言，我们界定了属于 Betaproteobacteria、Gammaproteobacteria、Bacilli、Actinobacteria、Flavobacteria 和 Alphaproteobacteria 的 74 个细菌分类群（操作分类单位或 OTU）。每个细菌群落在分类学上都很简单，平均包含 1.9 个常见（每个样本的相对丰度 > 5%）细菌 OTU。基于 16S rRNA 序列的最丰富和最普遍（出现在 92% 的样本中）的细菌 OTU 与之前描述的 Betaproteobacteria 物种 Snodgrassella alvi 非常匹配。首次在与蜜蜂相关的外部环境中描述的细菌在许多肠道细菌群落中占主导地位，这表明它们并不严格依赖于内部肠道环境。 ARISA 数据显示细菌群落结构与蜜蜂采样地理位置之间存在相关性，这表明至少一部分细菌物种可能是通过环境传播的。使用光学和荧光显微镜，我们证明肠道细菌在回肠内上皮表面形成生物膜，证实了从意大利蜜蜂获得的结果。
Gut bacterial communities of bumble bees are correlated with defense against pathogens. Further understanding this host-microbe association is vitally important as bumble bees are currently experiencing global population declines, potentially due in part to emergent diseases. In this study, we used pyrosequencing and community fingerprinting (ARISA) to characterize the gut microbial communities of nine bumble species from across the Bombus phylogeny. Overall, we delimited 74 bacterial taxa (operational taxonomic units or OTUs) belonging to Betaproteobacteria, Gammaproteobacteria, Bacilli, Actinobacteria, Flavobacteria and Alphaproteobacteria. Each bacterial community was taxonomically simple, containing an average of 1.9 common (relative abundance per sample > 5%) bacterial OTUs. The most abundant and prevalent (occurring in 92% of the samples) bacterial OTU, based on 16S rRNA sequences, closely matched that of the previously described Betaproteobacteria species Snodgrassella alvi. Bacteria that were first described in bee-related external environments dominated a number of gut bacterial communities, suggesting that they are not strictly dependent on the internal gut environment. The ARISA data showed a correlation between bacterial community structures and the geographic locations where the bees were sampled, suggesting that at least a subset of the bacterial species may be transmitted environmentally. Using light and fluorescent microscopy, we demonstrated that the gut bacteria form a biofilm on the internal epithelial surface of the ileum, corroborating results obtained from Apis mellifera.
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影响因子：
4
作者：
Fraune, Sebastian;Bosch, Thomas C. G.
通讯作者：
Bosch, Thomas C. G.
影响因子：
3.7
作者：
Anderson KE;Sheehan TH;Mott BM;Maes P;Snyder L;Schwan MR;Walton A;Jones BM;Corby-Harris V
通讯作者：
Corby-Harris V
DOI：
10.1099/ijs.0.023838-0
发表时间：
2011-04-01
影响因子：
2.8
作者：
Endo, Akihito;Irisawa, Tomohiro;Dicks, Leon M. T.
通讯作者：
Dicks, Leon M. T.
DOI：
10.1073/pnas.062067299
发表时间：
2002-04-02
影响因子：
11.1
作者：
Gil, R;Sabater-Muñoz, B;Moya, A
通讯作者：
Moya, A
影响因子：
4.2
作者：
Babendreier, Dirk;Joller, David;Widmer, Franco
通讯作者：
Widmer, Franco