Spatially and cell-type resolved quantitative proteomic atlas of healthy human skin.
Spatially and cell-type resolved quantitative proteomic atlas of healthy human skin.
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DOI：
10.1038/s41467-020-19383-8
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发表时间：
2020-11-05
影响因子：
16.6
通讯作者：
Mann M
中科院分区：
文献类型：
--
作者：
Dyring-Andersen B;Løvendorf MB;Coscia F;Santos A;Møller LBP;Colaço AR;Niu L;Bzorek M;Doll S;Andersen JL;Clark RA;Skov L;Teunissen MBM;Mann M
人类皮肤利用功能不同的层、细胞类型和细胞外基质提供物理完整性和免疫保护，免受外部环境的影响。尽管皮肤的组成蛋白在人类健康和疾病中发挥着核心作用，但尚未对其进行系统的表征。在这里，我们结合先进的组织解剖方法、流式细胞术和最先进的蛋白质组学来描述人类皮肤的空间分辨定量蛋白质组图谱。我们根据空间位置和细胞起源对 10,701 种蛋白质进行了定量。由此产生的蛋白质图谱和我们的初始数据分析证明了蛋白质组学对于了解皮肤内细胞类型多样性的价值。我们描述了结构蛋白、已知的和以前未描述的特定于细胞亚群的蛋白质以及具有特殊免疫功能的蛋白质（例如细胞因子和趋化因子）的定量分布。我们预计这个人类皮肤蛋白质组图谱将成为基础和转化研究的重要社区资源 (https://skin.science/)。人类皮肤是一个高度复杂的器官，包含多个组织层和不同的细胞类型。在这里，作者提出了健康人类皮肤的空间分辨定量蛋白质组图谱，描述了四个皮肤层和九种细胞类型的蛋白质谱。
Human skin provides both physical integrity and immunological protection from the external environment using functionally distinct layers, cell types and extracellular matrix. Despite its central role in human health and disease, the constituent proteins of skin have not been systematically characterized. Here, we combine advanced tissue dissection methods, flow cytometry and state-of-the-art proteomics to describe a spatially-resolved quantitative proteomic atlas of human skin. We quantify 10,701 proteins as a function of their spatial location and cellular origin. The resulting protein atlas and our initial data analyses demonstrate the value of proteomics for understanding cell-type diversity within the skin. We describe the quantitative distribution of structural proteins, known and previously undescribed proteins specific to cellular subsets and those with specialized immunological functions such as cytokines and chemokines. We anticipate that this proteomic atlas of human skin will become an essential community resource for basic and translational research (https://skin.science/). The human skin is a highly complex organ comprising multiple tissue layers and diverse cell types. Here, the authors present a spatially-resolved quantitative proteomic atlas of the healthy human skin, characterizing the protein profiles of four skin layers and nine cell types.
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