How hot are your ions in TWAVE ion mobility spectrometry?
How hot are your ions in TWAVE ion mobility spectrometry?
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DOI：
10.1007/s13361-011-0313-7
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发表时间：
2012-03
影响因子：
3.2
通讯作者：
Williams, Evan R.
中科院分区：
文献类型：
--
作者：
Merenbloom, Samuel I.;Flick, Tawnya G.;Williams, Evan R.
使用单质子化的亮氨酸脑啡肽二聚体作为化学温度计，通过监测二聚体解离成单体以及随后单体解离成a-、B-和y-离子，测量行波离子迁移谱（TWIMS）分析期间离子的有效温度，作为仪器参数的函数。在固定的氦池和TWIMS池气体流速下，解离的程度不随波速或波高显著变化，除了在不常用的低波速（<500 m/s）下。增加氮气进入TWIMS池的流速和降低氦气进入氦池的流速导致更大的解离。然而，由二聚体的解离形成的碎片离子的迁移率分布注入到TWIMS细胞后几乎是不可区分的碎片离子在TWIMS分析所有TWIMS实验之前，在碰撞细胞中形成的。这些结果表明，加热和解离发生时，离子注入到TWIMS电池，有效温度随后降低到一个点，在TWIMS分析过程中没有观察到进一步的解离。在180 mL/min的氦气流速、80 mL/min的TWIMS流速和40 V的行波高度下，TWIMS分析期间的有效离子温度的上限为449 K，这远低于先前报道的值。离子加热TWIMS的气相蛋白质构象的影响。
Effective temperatures of ions during traveling wave ion mobility spectrometry (TWIMS) analysis were measured using singly protonated leucine enkephalin dimer as a chemical thermometer by monitoring dissociation of the dimer into monomer, as well as the subsequent dissociation of monomer into a-, b-, and y-ions, as a function of instrumental parameters. At fixed helium cell and TWIMS cell gas flow rates, the extent of dissociation does not vary significantly with either the wave velocity or wave height, except at low (<500 m/s) wave velocities that are not commonly used. Increasing the flow rate of nitrogen gas into the TWIMS cell and decreasing the flow rate of helium gas into the helium cell resulted in greater dissociation. However, the mobility distributions of the fragment ions formed by dissociation of the dimer upon injection into the TWIMS cell are nearly indistinguishable from those of fragment ions formed in the collision cell prior to TWIMS analysis for all TWIMS experiments. These results indicate that heating and dissociation occur when ions are injected into the TWIMS cell, and that the effective temperature subsequently decreases to a point at which no further dissociation is observed during the TWIMS analysis. An upper limit to the effective ion temperature of 449 K during TWIMS analysis is obtained at a helium flow rate of 180 mL/min, TWIMS flow rate of 80 mL/min and traveling wave height of 40 V, which is well below previously reported values. Effects of ion heating in TWIMS on gas-phase protein conformation are presented.
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影响因子：
2
作者：
Giles, Kevin;Williams, Jonathan P.;Campuzano, Iain
通讯作者：
Campuzano, Iain
影响因子：
1.3
作者：
COHEN, MJ;KARASEK, FW
通讯作者：
KARASEK, FW
影响因子：
7.4
作者：
Badman, ER;Hoaglund-Hyzer, CS;Clemmer, DE
通讯作者：
Clemmer, DE
影响因子：
7.4
作者：
Hoaglund, CS;Valentine, SJ;Clemmer, DE
通讯作者：
Clemmer, DE
影响因子：
7.4
作者：
Lee, YJ;Hoaglund-Hyzer, CS;Clemmer, DE
通讯作者：
Clemmer, DE