A periosteum-derived cell line to study the role of BMP/TGFβ signaling in periosteal cell behavior and function.
A periosteum-derived cell line to study the role of BMP/TGFβ signaling in periosteal cell behavior and function.
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DOI：
10.3389/fphys.2023.1221152
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发表时间：
2023
影响因子：
4
通讯作者：
中科院分区：
文献类型：
--
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骨膜是围绕每个骨骼元件的薄组织，其含有参与骨发育、出生后附着骨生长、负荷诱导的骨形成和骨折修复的干细胞和祖细胞。BMP和TGFβ信号传导对于骨膜活性和骨膜细胞行为是重要的，但是由于与原代骨膜细胞分离和体外实验相关的限制，缺乏对这些途径对驻留在骨膜中的特定细胞群的影响的彻底检查。在这里，我们描述了一种新的骨膜衍生的克隆细胞（PDC）线从出生后第14天的小鼠，并使用它来检查骨膜细胞在体外的行为。PDCs表现出在骨骼发育、维持和骨修复过程中观察到的骨膜细胞的关键特征。具体而言，PDCs表达已建立的骨膜标志物，可在培养物中扩增，表现出分化成软骨细胞、成骨细胞和脂肪细胞的能力，并表现出对物理刺激的成骨反应。当用活化配体BMP 2和TGFβ-1处理时，PDC还参与BMP和/或TGFβ信号传导，并响应于经由流体剪切的机械刺激。我们相信，这种PDC线将是有用的大规模，长期的实验是不可行的，当使用原代骨膜细胞。预期未来的用途包括推进我们对在附着骨生长和骨折修复过程中发生的信号相互作用的理解，以及开发药物筛选平台以发现新的生长和骨折愈合因子。
The periosteum is a thin tissue surrounding each skeletal element that contains stem and progenitor cells involved in bone development, postnatal appositional bone growth, load-induced bone formation, and fracture repair. BMP and TGFβ signaling are important for periosteal activity and periosteal cell behavior, but thorough examination of the influence of these pathways on specific cell populations resident in the periosteum is lacking due to limitations associated with primary periosteal cell isolations and in vitro experiments. Here we describe the generation of a novel periosteum-derived clonal cell (PDC) line from postnatal day 14 mice and use it to examine periosteal cell behavior in vitro. PDCs exhibit key characteristics of periosteal cells observed during skeletal development, maintenance, and bone repair. Specifically, PDCs express established periosteal markers, can be expanded in culture, demonstrate the ability to differentiate into chondrocytes, osteoblasts, and adipocytes, and exhibit an osteogenic response to physical stimulation. PDCs also engage in BMP and/or TGFβ signaling when treated with the activating ligands BMP2 and TGFβ-1, and in response to mechanical stimulation via fluid shear. We believe that this PDC line will be useful for large-scale, long-term experiments that were not feasible when using primary periosteal cells. Anticipated future uses include advancing our understanding of the signaling interactions that occur during appositional bone growth and fracture repair and developing drug screening platforms to discover novel growth and fracture healing factors.
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影响因子：
5.2
作者：
Grcevic, Danka;Pejda, Slavica;Matthews, Brya G.;Repic, Dario;Wang, Liping;Li, Haitao;Kronenberg, Mark S.;Jiang, Xi;Maye, Peter;Adams, Douglas J.;Rowe, David W.;Aguila, Hector L.;Kalajzic, Ivo
通讯作者：
Kalajzic, Ivo
影响因子：
4.1
作者：
Arnsdorf, Emily J.;Jones, Luis M.;Jacobs, Christopher R.
通讯作者：
Jacobs, Christopher R.
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影响因子：
3.8
作者：
Brown, Sarah;Malik, Saif;Aljammal, Maria;O'Flynn, Aine;Hobbs, Carl;Shah, Mittal;Roberts, Scott J.;Logan, Malcolm P. O.
通讯作者：
Logan, Malcolm P. O.
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影响因子：
5.5
作者：
Lademann F;Hofbauer LC;Rauner M
通讯作者：
Rauner M
DOI：
10.1002/jbmr.2140
发表时间：
2014
期刊：
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
影响因子：
--
作者：
Matthews BG;Grcevic D;Wang L;Hagiwara Y;Roguljic H;Joshi P;Shin DG;Adams DJ;Kalajzic I
通讯作者：
Kalajzic I